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<p>Turmeric, referred to as “Golden Spice of India,” belongs to <i>Zingiberaceae</i> family is cultivated in South-eastern and North-eastern regions of India, where it displays the regional diversity. In this study, turmeric rhizomes of <i>Curcuma longa</i> (yellow turmeric) from the South-eastern India and <i>Curcuma caesia</i> (black turmeric) from North-eastern India were collected. Proteins from these rhizome samples were extracted by using four different pH based buffer systems. The sequentially purified peptide filtrates obtained through ultrafiltration with 10&#xa0;kDa and 3&#xa0;kDa cut off membranes were quantified and assessed for their antioxidant potential by FRAP, NOS, DPPH and ABTS assays. The results showed that the highest antioxidant activities were observed in the Phosphate (pH 7.0) and Tris-HCl (pH 8.0) buffers. Further analysis of the 3&#xa0;kDa filtrates from both turmeric samples by using HPLC confirmed the presence of peptides, which were subsequently identified by MALDI-TOF MS. The peptides identified in this study exhibited the promising bioactive potential based on their sequences and showed the less cleaving potential evaluated by <i>insilico</i> peptideCutter analysis. Future research will focus on validating these activities through comprehensive experimental approaches, including cell-based antioxidant, mechanistic studies, and antimicrobial evaluations. Such investigations will be essential to confirm their biological relevance and to determine its suitability for nutraceutical applications.</p>

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Evaluation of antioxidant activities of bioactive peptides extracted from Curcuma longa and Curcuma caesia from South-eastern and North-Eastern India

  • 期刊论文

  • 发表日期：

    2026年2月4日

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  • Anshula Narayanasamy,
  • Abinaya Kanagaraja,
  • Munusamy Thirumavalavan,
  • Meenakumari Sakthivelu,
  • Raman Pachaiappan

摘要

Turmeric, referred to as “Golden Spice of India,” belongs to Zingiberaceae family is cultivated in South-eastern and North-eastern regions of India, where it displays the regional diversity. In this study, turmeric rhizomes of Curcuma longa (yellow turmeric) from the South-eastern India and Curcuma caesia (black turmeric) from North-eastern India were collected. Proteins from these rhizome samples were extracted by using four different pH based buffer systems. The sequentially purified peptide filtrates obtained through ultrafiltration with 10 kDa and 3 kDa cut off membranes were quantified and assessed for their antioxidant potential by FRAP, NOS, DPPH and ABTS assays. The results showed that the highest antioxidant activities were observed in the Phosphate (pH 7.0) and Tris-HCl (pH 8.0) buffers. Further analysis of the 3 kDa filtrates from both turmeric samples by using HPLC confirmed the presence of peptides, which were subsequently identified by MALDI-TOF MS. The peptides identified in this study exhibited the promising bioactive potential based on their sequences and showed the less cleaving potential evaluated by insilico peptideCutter analysis. Future research will focus on validating these activities through comprehensive experimental approaches, including cell-based antioxidant, mechanistic studies, and antimicrobial evaluations. Such investigations will be essential to confirm their biological relevance and to determine its suitability for nutraceutical applications.