Scholarlink

<p>Sepsis caused by Gram-negative bacteria such as <i>Escherichia coli</i> and <i>Salmonella enterica</i> is a major contributor to global morbidity and mortality. This study investigated the protective effects of four human milk-derived probiotic strains, <i>Staphylococcus hominis</i>, <i>Staphylococcus epidermidis</i>, <i>Streptococcus salivarius</i>, and <i>Streptococcus lactarius</i>, in murine models of pathogen- and lipopolysaccharide (LPS)-induced sepsis. Mice received probiotic pre-treatment before challenge with LPS, <i>E. coli</i>, or <i>Salmonella enterica</i> typhimurium. Clinical signs, survival, body weight, gross and histopathological lesions, serum biochemical markers, fecal bacterial load, and intestinal cytokine gene expression (TNF-α, IL-6) were recorded. Pathogen- and LPS-challenged groups showed high mortality, significant weight loss, marked intestinal pathology, elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, and creatinine, increased fecal pathogen counts, and upregulated TNF-α and IL-6 expression. Probiotic pre-treatment improved survival, preserved body weight, reduced lesion severity, normalized biochemical parameters, lowered fecal pathogen load, and downregulated pro-inflammatory cytokines. Among the tested strains, <i>Strep. lactarius</i> provided the most consistent protection in <i>E. coli</i> and LPS models, whereas <i>Strep. Salivarius</i> was most effective against <i>Salmonella</i>-induced sepsis. The results indicate that specific human milk-derived probiotics can reduce systemic inflammation and organ injury in Gram-negative bacterial sepsis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Assessment of Protective Role of Different Probiotic Strains against Bacterial and Endotoxin-Induced Sepsis in a Mouse Model

  • 期刊论文

  • 发表日期：

    2025年12月27日

查看全文
  • Sabeeka Sajjal,
  • Hasnat Ahmad Bilal,
  • Ambreen Zahra,
  • Ayesha Saeed,
  • Aiman Khan,
  • Muhammad Musa Mubashar,
  • Aisha Khatoon,
  • Muhammad Imran Arshad

摘要

Sepsis caused by Gram-negative bacteria such as Escherichia coli and Salmonella enterica is a major contributor to global morbidity and mortality. This study investigated the protective effects of four human milk-derived probiotic strains, Staphylococcus hominis, Staphylococcus epidermidis, Streptococcus salivarius, and Streptococcus lactarius, in murine models of pathogen- and lipopolysaccharide (LPS)-induced sepsis. Mice received probiotic pre-treatment before challenge with LPS, E. coli, or Salmonella enterica typhimurium. Clinical signs, survival, body weight, gross and histopathological lesions, serum biochemical markers, fecal bacterial load, and intestinal cytokine gene expression (TNF-α, IL-6) were recorded. Pathogen- and LPS-challenged groups showed high mortality, significant weight loss, marked intestinal pathology, elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, and creatinine, increased fecal pathogen counts, and upregulated TNF-α and IL-6 expression. Probiotic pre-treatment improved survival, preserved body weight, reduced lesion severity, normalized biochemical parameters, lowered fecal pathogen load, and downregulated pro-inflammatory cytokines. Among the tested strains, Strep. lactarius provided the most consistent protection in E. coli and LPS models, whereas Strep. Salivarius was most effective against Salmonella-induced sepsis. The results indicate that specific human milk-derived probiotics can reduce systemic inflammation and organ injury in Gram-negative bacterial sepsis.