Scholarlink

<p>The global rise of multidrug-resistant (MDR) pathogens, particularly <i>Staphylococcus aureus</i>, necessitates the development of novel antimicrobial approaches. Peptidoglycan Recognition Protein-S (PGRP-S), an innate immune effector, is known to target bacterial cell walls by recognizing and hydrolyzing peptidoglycans. Camel (<i>Camelus dromedarius</i>) milk, renowned for its medicinal properties, is a rich source of PGRP-S and contributes to neonatal immunity. This study investigates the antimicrobial and immunomodulatory effects of camel milk-derived PGRP-S (C-PGRP-S) against <i>S. aureus</i>. Purified C-PGRP-S exhibited potent antibacterial activity, completely inhibiting bacterial growth at 40&#xa0;μg/mL in MIC assays and demonstrating rapid bactericidal action in time-kill kinetics. Scanning electron microscopy revealed dose-dependent morphological damage to bacterial membranes. Immunologically, C-PGRP-S suppressed the overproduction of pro-inflammatory cytokines and restored ROS production in M1 macrophages significantly in infected cells. These results suggest that C-PGRP-S operates via a dual mechanism: direct bacterial lysis and immune modulation. Structural insights from the crystallographic model of camel PGRP-S (PDB ID: 3NW3) further highlight its molecular interaction with peptidoglycans. Taken together, C-PGRP-S emerges as a promising macromolecular therapeutic candidate, with potential as a natural alternative or adjunct to existing antibiotics in managing drug-resistant <i>S. aureus</i> infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Peptidoglycan Recognition Protein-S as a Dual-Action Antimicrobial and Immunomodulatory Agent Against Staphylococcus aureus

  • 期刊论文

  • 发表日期：

    2025年11月18日

查看全文
  • Priya Verma,
  • Priyanka Swaroop,
  • Surabhi Pandit,
  • Ved Prakash,
  • Surender Kumar Sharawat,
  • T. P. Singh,
  • Sujata Sharma,
  • Pradeep Sharma

摘要

The global rise of multidrug-resistant (MDR) pathogens, particularly Staphylococcus aureus, necessitates the development of novel antimicrobial approaches. Peptidoglycan Recognition Protein-S (PGRP-S), an innate immune effector, is known to target bacterial cell walls by recognizing and hydrolyzing peptidoglycans. Camel (Camelus dromedarius) milk, renowned for its medicinal properties, is a rich source of PGRP-S and contributes to neonatal immunity. This study investigates the antimicrobial and immunomodulatory effects of camel milk-derived PGRP-S (C-PGRP-S) against S. aureus. Purified C-PGRP-S exhibited potent antibacterial activity, completely inhibiting bacterial growth at 40 μg/mL in MIC assays and demonstrating rapid bactericidal action in time-kill kinetics. Scanning electron microscopy revealed dose-dependent morphological damage to bacterial membranes. Immunologically, C-PGRP-S suppressed the overproduction of pro-inflammatory cytokines and restored ROS production in M1 macrophages significantly in infected cells. These results suggest that C-PGRP-S operates via a dual mechanism: direct bacterial lysis and immune modulation. Structural insights from the crystallographic model of camel PGRP-S (PDB ID: 3NW3) further highlight its molecular interaction with peptidoglycans. Taken together, C-PGRP-S emerges as a promising macromolecular therapeutic candidate, with potential as a natural alternative or adjunct to existing antibiotics in managing drug-resistant S. aureus infections.