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<p>Lactic acid bacteria (LAB) play a crucial role in food fermentation and human health. This study introduces <i>Lactiplantibacillus plantarum </i>(<i>L. plantarum</i>) QL-19, a novel probiotic strain isolated from traditional Hunan pickles and characterized via whole-genome sequencing and in vitro functional assays. The 3,396,702 genome contains 3236 predicted coding sequences, including genes associated with stress tolerance, adhesion, and cholesterol metabolism (<i>cbh</i>, <i>cfa</i>, and hydroxymethylglutaryl-CoA synthase). Genomic analysis further revealed biosynthetic gene clusters encoding antimicrobial and antioxidant compounds, emphasizing its functional versatility. A comprehensive safety assessment confirmed that QL-19 is non-pathogenic and non-hemolytic, meeting probiotic safety standards. In HepG2 cell–based experiments, <i>L. plantarum</i> QL-19 reduced intracellular total cholesterol levels. RT-qPCR and Western blotting were employed to evaluate the expression of genes and proteins involved in cholesterol metabolism. The results demonstrated that QL-19 enhanced cholesterol metabolism by upregulating the expression of SREBP2, LDLR, and CYP7A1, while downregulating NPC1L1 and HMGCR. The results suggest that QL-19 facilitates cholesterol metabolism through multiple pathways. Taken together with its 75.4% in vitro cholesterol degradation rate, these findings highlight the potential of QL-19 as a cholesterol-lowering probiotic candidate for cardiovascular health applications.</p>

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Characterization of the Probiotic Properties and Cholesterol Metabolism Regulation Potential of Lactiplantibacillus plantarum QL-19

  • 期刊论文

  • 发表日期：

    2025年8月29日

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  • Mingkai Liu,
  • Yanlei Han,
  • Shanshan Wang,
  • Yiming Wang,
  • Hui Xu

摘要

Lactic acid bacteria (LAB) play a crucial role in food fermentation and human health. This study introduces Lactiplantibacillus plantarum (L. plantarum) QL-19, a novel probiotic strain isolated from traditional Hunan pickles and characterized via whole-genome sequencing and in vitro functional assays. The 3,396,702 genome contains 3236 predicted coding sequences, including genes associated with stress tolerance, adhesion, and cholesterol metabolism (cbh, cfa, and hydroxymethylglutaryl-CoA synthase). Genomic analysis further revealed biosynthetic gene clusters encoding antimicrobial and antioxidant compounds, emphasizing its functional versatility. A comprehensive safety assessment confirmed that QL-19 is non-pathogenic and non-hemolytic, meeting probiotic safety standards. In HepG2 cell–based experiments, L. plantarum QL-19 reduced intracellular total cholesterol levels. RT-qPCR and Western blotting were employed to evaluate the expression of genes and proteins involved in cholesterol metabolism. The results demonstrated that QL-19 enhanced cholesterol metabolism by upregulating the expression of SREBP2, LDLR, and CYP7A1, while downregulating NPC1L1 and HMGCR. The results suggest that QL-19 facilitates cholesterol metabolism through multiple pathways. Taken together with its 75.4% in vitro cholesterol degradation rate, these findings highlight the potential of QL-19 as a cholesterol-lowering probiotic candidate for cardiovascular health applications.