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<p><i>Spodoptera frugiperda</i> (JE Smith, 1797) is a polyphagous pest within the Noctuidae family, affecting over 120 plant species and mostly inflicting damage on the Poaceae family in India. The present work investigated the oral delivery of dsRNA to second instar <i>S. frugiperda</i> larvae through leaf feeding application. The dsRNA was engineered to suppress three vacuolar-ATPase (vATPase) genes, namely <i>vATPase-A, vATPase-D</i> and <i>vATPase-E</i>. Transcript expression was evaluated via qRT-PCR to assess the efficacy of RNAi. The leaf feeding bioassay with five different concentrations (10, 20, 30, 40, and 50&#xa0;µg/cm<sup>2</sup>/leaf) of <i>vATPase-A</i>, <i>vATPase-D</i> and <i>vATPase-E</i> dsRNAs produced a dose-dependent larval death rate between 45% to 85.0%. The larvae that consumed vATPase dsRNA treated maize leaves exhibited a significant reduction in the expression of <i>vATPase-A</i>, followed by <i>vATPase-D</i> and <i>vATPase-E</i>. Regarding treatments, leaf feeding damage was diminished at 72&#xa0;h post ingestion. According to the study's overall findings, <i>vATPase-A</i> and <i>vATPase-D</i> gene silencing may offer a practical method of future <i>S. frugiperda</i> control.</p>

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Gene silencing strategy for fall armyworm (Spodoptera frugiperda) control utilizing dsRNA targeting vATPase

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Ranganathan Muthusamy,
  • Govindaraju Ramkumar,
  • Sengottayan Senthil-Nathan,
  • Narayanasamy Prabhu,
  • Naraayanan Madhiyalagan

摘要

Spodoptera frugiperda (JE Smith, 1797) is a polyphagous pest within the Noctuidae family, affecting over 120 plant species and mostly inflicting damage on the Poaceae family in India. The present work investigated the oral delivery of dsRNA to second instar S. frugiperda larvae through leaf feeding application. The dsRNA was engineered to suppress three vacuolar-ATPase (vATPase) genes, namely vATPase-A, vATPase-D and vATPase-E. Transcript expression was evaluated via qRT-PCR to assess the efficacy of RNAi. The leaf feeding bioassay with five different concentrations (10, 20, 30, 40, and 50 µg/cm2/leaf) of vATPase-A, vATPase-D and vATPase-E dsRNAs produced a dose-dependent larval death rate between 45% to 85.0%. The larvae that consumed vATPase dsRNA treated maize leaves exhibited a significant reduction in the expression of vATPase-A, followed by vATPase-D and vATPase-E. Regarding treatments, leaf feeding damage was diminished at 72 h post ingestion. According to the study's overall findings, vATPase-A and vATPase-D gene silencing may offer a practical method of future S. frugiperda control.