<p>Root-knot nematodes (<i>Meloidogyne</i> spp.) are significant pests of vegetable crops, particularly in the tropics and subtropics. All commercial okra varieties are susceptible to these nematodes, and yield losses of up to 19.5% have been documented in okra. The objective of this study was to identify resistant genotypes to help our breeders with gene modification. This study was conducted to evaluate seventy genotypes of okra (63 <i>Abelmoschus moschatus</i> accessions and 7 <i>Abelmoschus esculentus</i> cultivars) against <i>Meloidogyne incognita</i>. Five accessions of <i>A. moschatus</i> EC3598268, EC359836, EC360672, EC360900 and EC361129 exhibited moderate resistance, with a root gall index of 2 and minimal reduction in plant growth parameters than susceptible genotypes. A positive relationship was demonstrated between number of root galls per plant and reduction in plant growth parameters. During our study, none of the tested genotypes were observed to be immune or resistant to nematode infection. Efforts were made to develop interspecific F₁ hybrids between root-knot nematode susceptible and some moderately resistant parents, but none of these attempts resulted in successful germination of interspecific F₁ plants. This implies a lack of compatibility between these parents for crossbreeding. Biochemical analysis aimed at assessing root-knot nematode resistance indicated that the activity of biochemical and antioxidant traits was significantly higher in resistant genotypes compared to their susceptible counterparts. This trend was consistent between both inoculated and uninoculated sets of genotypes. This suggests that elevated levels of these parameters are associated with resistance to root-knot nematode.</p>