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<p>n-3 long-chain (≥ C<sub>20</sub>) polyunsaturated fatty acids (n-3 LC-PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are essential nutrients found in fish oils at high concentrations. While fish LC-PUFA synthesis enzymatic activity was examined using a yeast heterologous expression system, this does not necessarily reflect LC-PUFA synthesis de novo activity. In this study, n-3 LC-PUFA synthesis activity was evaluated for fish cell lines from marine, anadromous, catadromous, and freshwater habitats by combinatorial analysis using deuterium-labeled PUFA with liquid chromatography–electrospray ionization tandem mass spectrometry (LC-ESI MS/MS). Each fish cell line converted deuterium-labeled PUFA to deuterium-labeled LC-PUFA depending on the activity of the available LC-PUFA synthetic enzymes. To further investigate de novo LC-PUFA synthesis activity in fish, enzymatic synthesis activity was confirmed between cell lines and fish tissues using fish hepatocytes. This approach is highly effective in evaluating and comparing LC-PUFA synthesis activities among cell lines and fish tissues.</p>

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Evaluation of the n-3 PUFA synthetic ability of cell lines established from fish living in diverse water habitats

  • 期刊论文

  • 发表日期：

    2025年1月30日

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  • Hayato Nyunoya,
  • Rio Kawagoe,
  • Yohei Ishibashi,
  • Nozomu Okino

摘要

n-3 long-chain (≥ C20) polyunsaturated fatty acids (n-3 LC-PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are essential nutrients found in fish oils at high concentrations. While fish LC-PUFA synthesis enzymatic activity was examined using a yeast heterologous expression system, this does not necessarily reflect LC-PUFA synthesis de novo activity. In this study, n-3 LC-PUFA synthesis activity was evaluated for fish cell lines from marine, anadromous, catadromous, and freshwater habitats by combinatorial analysis using deuterium-labeled PUFA with liquid chromatography–electrospray ionization tandem mass spectrometry (LC-ESI MS/MS). Each fish cell line converted deuterium-labeled PUFA to deuterium-labeled LC-PUFA depending on the activity of the available LC-PUFA synthetic enzymes. To further investigate de novo LC-PUFA synthesis activity in fish, enzymatic synthesis activity was confirmed between cell lines and fish tissues using fish hepatocytes. This approach is highly effective in evaluating and comparing LC-PUFA synthesis activities among cell lines and fish tissues.