<p>Prosthetic limbs are essential for individuals with amputations, enabling mobility and supporting daily activities, including a return to work. However, altered gait patterns often result from amputees’ reluctance to fully rely on their prostheses for weight-bearing during walking. These unique gait characteristics must be carefully considered in the design and control of electronically controlled hydraulic prosthetic knees. This study proposes a novel evaluation method for hydraulic cylinders in prosthetic knees and a simulation framework for their control. A kinematic model of the prosthetic leg was developed to convert knee angle trajectories during level-ground walking into the displacement and velocity of the hydraulic cylinder piston. These parameters were applied to a material testing machine to analyze the damping characteristics of the hydraulic cylinder under varying nozzle angles. Furthermore, a gait simulation using a material testing machine was introduced to avoid the risks associated with direct patient testing. The proposed approach establishes initial setting values for hydraulic cylinders and provides a standardized protocol to evaluate their dynamic durability. These findings are expected to advance the development of electronically controlled prosthetic knees, improving their adaptability to the diverse needs of amputees.</p>