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<p>Phospholipase D (PLD)–mediated lipid signaling has been implicated in plant development and stress responses; however, its direct genetic role in plant reproduction remains poorly understood. In this study, we investigated the function of <i>OsPLDα2</i>, a rice homolog of maize ZmPLD3, in pollen tube growth and fertilization. Phylogenetic and domain analyses revealed that <i>OsPLDα2</i> belongs to the C2-PLD subfamily and contains a conserved C2 domain and two HKD catalytic motifs. Expression analyses showed that <i>OsPLDα2</i> is highly expressed in mature pollen. Subcellular localization showed its association with cytoskeletal and membrane systems. Using CRISPR/Cas9-mediated gene editing, we generated <i>ospldα2</i> knockout mutants and found that they exhibited normal vegetative growth but significantly reduced fertility. Cytological analyses revealed that <i>ospldα2</i> pollen displayed reduced germination rates, abnormal pollen tube elongation both in in vitro and in vivo. In addition, phalloidin staining showed altered F-actin labeling patterns in mutant pollen tubes, suggesting an association between <i>OsPLDα2</i> function and cytoskeletal organization during pollen tube growth. Our study provides direct genetic evidence linking PLD-mediated lipid signaling to pollen function in a monocot species and highlights the importance of PLD in reproductive development beyond its established roles in stress responses.</p>

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OsPLDα2 Contributes to Pollen Tube Growth and F-Actin Organization in Rice

  • 期刊论文

  • 发表日期：

    2026年7月23日

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  • Ji-Yun Lee,
  • Ga-Young Noh,
  • Ji-Hyun Kim,
  • Woo-Jong Hong,
  • Sun Tae Kim,
  • Wanqi Liang,
  • Yu-Jin Kim

摘要

Phospholipase D (PLD)–mediated lipid signaling has been implicated in plant development and stress responses; however, its direct genetic role in plant reproduction remains poorly understood. In this study, we investigated the function of OsPLDα2, a rice homolog of maize ZmPLD3, in pollen tube growth and fertilization. Phylogenetic and domain analyses revealed that OsPLDα2 belongs to the C2-PLD subfamily and contains a conserved C2 domain and two HKD catalytic motifs. Expression analyses showed that OsPLDα2 is highly expressed in mature pollen. Subcellular localization showed its association with cytoskeletal and membrane systems. Using CRISPR/Cas9-mediated gene editing, we generated ospldα2 knockout mutants and found that they exhibited normal vegetative growth but significantly reduced fertility. Cytological analyses revealed that ospldα2 pollen displayed reduced germination rates, abnormal pollen tube elongation both in in vitro and in vivo. In addition, phalloidin staining showed altered F-actin labeling patterns in mutant pollen tubes, suggesting an association between OsPLDα2 function and cytoskeletal organization during pollen tube growth. Our study provides direct genetic evidence linking PLD-mediated lipid signaling to pollen function in a monocot species and highlights the importance of PLD in reproductive development beyond its established roles in stress responses.