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<p>Dehydration-responsive element-binding (DREB) transcription factors mediate plant responses to abiotic stress by activating stress-inducible genes through an ABA-independent pathway. Although <i>OsDREB1</i> genes in rice are associated with cold, drought, and salinity tolerance, their roles in thermotolerance remain unclear. Therefore, this study aims to perform a genome-wide analysis of <i>OsDREB1</i> family members under heat stress using an integrative approach combining meta-expression profiling, qRT-PCR validation, protein–protein interaction network analysis, and heterologous overexpression assays in Arabidopsis. Expression profiling revealed distinct heat-responsive patterns among paralogs, and <i>OsDREB1F</i> showed the strongest and most rapid induction. Network analysis revealed that <i>OsDREB1</i>-related modules integrate early signaling with sustained transcriptional responses under heat stress. Functional assays in heterologous Arabidopsis demonstrated that <i>OsDREB1F</i> overexpression conferred the greatest improvement in post-stress survival rate, while <i>OsDREB1C</i> and <i>OsDREB1A</i> exhibited moderate and minimal effects, respectively. Collectively, these findings reveal heat-responsive <i>OsDREB1</i> genes and their potential interactors in rice, and further establish <i>OsDREB1F</i> as a key regulator of thermotolerance in Arabidopsis, highlighting <i>OsDREB1F</i> as a promising candidate for future functional validation in rice and for heat-resilience engineering strategies.</p>

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Genome-wide Analysis of Rice OsDREB1 Genes Under Heat Stress Reveals Functional Specialization of OsDREB1F for Thermotolerance in Arabidopsis

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • Sang Dae Yun,
  • Myung-Hee Kim,
  • Hye Lin Kim,
  • Je Yeon Choi,
  • Sung-Aeong Oh,
  • Jeong Hoe Kim,
  • Moon-Soo Soh,
  • Ki-Hong Jung,
  • Soon Ki Park

摘要

Dehydration-responsive element-binding (DREB) transcription factors mediate plant responses to abiotic stress by activating stress-inducible genes through an ABA-independent pathway. Although OsDREB1 genes in rice are associated with cold, drought, and salinity tolerance, their roles in thermotolerance remain unclear. Therefore, this study aims to perform a genome-wide analysis of OsDREB1 family members under heat stress using an integrative approach combining meta-expression profiling, qRT-PCR validation, protein–protein interaction network analysis, and heterologous overexpression assays in Arabidopsis. Expression profiling revealed distinct heat-responsive patterns among paralogs, and OsDREB1F showed the strongest and most rapid induction. Network analysis revealed that OsDREB1-related modules integrate early signaling with sustained transcriptional responses under heat stress. Functional assays in heterologous Arabidopsis demonstrated that OsDREB1F overexpression conferred the greatest improvement in post-stress survival rate, while OsDREB1C and OsDREB1A exhibited moderate and minimal effects, respectively. Collectively, these findings reveal heat-responsive OsDREB1 genes and their potential interactors in rice, and further establish OsDREB1F as a key regulator of thermotolerance in Arabidopsis, highlighting OsDREB1F as a promising candidate for future functional validation in rice and for heat-resilience engineering strategies.