Scholarlink

<p>ER Bodies accumulate atypical myrosinases that hydrolyzes glucosinolates, generating chemically reactive products toxic to pathogens and herbivores. NAI2 is an ER Body component that facilitates ER body formation, similar to the ER Body, its homologous proteins are shown only in Brassicaceae. A new type of endoplasmic reticulum-derived vesicles, NAIP vesicles—which are related to the ER Body—have been found in recent years, and NAIPs (NAI2-interact proteins) not only play a critical and redundant role in ER Body formation, but also could assemble other proteins to coordinate or promote cargo recruitment to drive the formation of the NAIP vesicle. Unlike NAI2, homologous proteins of NAIPs are shown in all kinds of plants with 3 or 4 members, which increases the difficulty in studying the biological function of NAIP vesicles. Here, we optimized the CRISPR/Cas9 system for concurrent editing of multiple target genes by designing suitable multiple targeting sequences and ingeniously utilizing enzymatic sites for the linkage of various promoters. Additionally, appropriate methodologies were employed to construct CRISPR/Cas9 expression vectors and introduce them into tomato, resulting in knocking off three members of the NAIP vesicle-associated gene family. Noteworthy, this CRISPR/Cas9 system has potential applications for functional studies of the functionally redundant genes in eudicot plants. We also found that <i>slnaip1/2/3s</i> mutants were shorter in the youth age compared to the wild type, which provides a favorable clue for the subsequent identification of NAIP vesicles in other plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Knock Off 3 SlNAIP Genes with Modified CRISPR-Cas9 Causes Dwarf Phenotype in Tomato

  • 期刊论文

  • 发表日期：

    2025年3月14日

查看全文
  • Jia Liu,
  • Xinya Dan,
  • Lili Wu,
  • Pingping Fang,
  • Yan Zhang,
  • Xifeng Li,
  • Yanfei Ding

摘要

ER Bodies accumulate atypical myrosinases that hydrolyzes glucosinolates, generating chemically reactive products toxic to pathogens and herbivores. NAI2 is an ER Body component that facilitates ER body formation, similar to the ER Body, its homologous proteins are shown only in Brassicaceae. A new type of endoplasmic reticulum-derived vesicles, NAIP vesicles—which are related to the ER Body—have been found in recent years, and NAIPs (NAI2-interact proteins) not only play a critical and redundant role in ER Body formation, but also could assemble other proteins to coordinate or promote cargo recruitment to drive the formation of the NAIP vesicle. Unlike NAI2, homologous proteins of NAIPs are shown in all kinds of plants with 3 or 4 members, which increases the difficulty in studying the biological function of NAIP vesicles. Here, we optimized the CRISPR/Cas9 system for concurrent editing of multiple target genes by designing suitable multiple targeting sequences and ingeniously utilizing enzymatic sites for the linkage of various promoters. Additionally, appropriate methodologies were employed to construct CRISPR/Cas9 expression vectors and introduce them into tomato, resulting in knocking off three members of the NAIP vesicle-associated gene family. Noteworthy, this CRISPR/Cas9 system has potential applications for functional studies of the functionally redundant genes in eudicot plants. We also found that slnaip1/2/3s mutants were shorter in the youth age compared to the wild type, which provides a favorable clue for the subsequent identification of NAIP vesicles in other plants.