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<p>As secretory vesicle-residing soluble <i>N</i>-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), VESICLE-ASSOCIATED MEMBRANE PROTEIN 721 and 722 (VAMP721/722) are required for stress responses as well as growth and development in plants. We previously reported the post-translational downregulation of VAMP721/722 abundance by the plant abiotic stress hormone abscisic acid (ABA) in Arabidopsis. However, how ABA induces VAMP721/722 degradation in plants is yet unclear. Here, we show that the SLOW MOTION (SLOMO) F-box protein mediates the ABA-induced degradation of VAMP721/722 in Arabidopsis. We detected higher VAMP721/722 levels in <i>slomo-1</i> missense and <i>slomo-2</i> null mutants than wild-type (WT) plants. We also found that VAMP722 interacts with the Leu-rich repeat (LRR) domain of SLOMO, but not with the variant LRR domain in <i>slomo-1</i> mutant, in both in vitro and <i>in planta</i> assays. The significantly elevated growth in <i>slomo</i> plants compared to WT under ABA and abiotic stresses implies that plants may manage abiotic stress responses by regulating VAMP721/722 levels at least in part via SLOMO.</p>

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The SLOMO F-Box Protein is Required for ABA-Induced Degradation of VAMP721/722 in Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年1月7日

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  • Yunjin Choi,
  • Hani Kim,
  • Hyeokjin Kwon,
  • Hyera Jung,
  • Arim Kim,
  • Sujin Kim,
  • Sunglan Chung,
  • Taijoon Chung,
  • Jae-Hoon Lee,
  • Hye Sup Yun,
  • Chian Kwon

摘要

As secretory vesicle-residing soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), VESICLE-ASSOCIATED MEMBRANE PROTEIN 721 and 722 (VAMP721/722) are required for stress responses as well as growth and development in plants. We previously reported the post-translational downregulation of VAMP721/722 abundance by the plant abiotic stress hormone abscisic acid (ABA) in Arabidopsis. However, how ABA induces VAMP721/722 degradation in plants is yet unclear. Here, we show that the SLOW MOTION (SLOMO) F-box protein mediates the ABA-induced degradation of VAMP721/722 in Arabidopsis. We detected higher VAMP721/722 levels in slomo-1 missense and slomo-2 null mutants than wild-type (WT) plants. We also found that VAMP722 interacts with the Leu-rich repeat (LRR) domain of SLOMO, but not with the variant LRR domain in slomo-1 mutant, in both in vitro and in planta assays. The significantly elevated growth in slomo plants compared to WT under ABA and abiotic stresses implies that plants may manage abiotic stress responses by regulating VAMP721/722 levels at least in part via SLOMO.