<p>The use of building stones in constructions reflects a region’s historical, social, and cultural aspects. One notable example is Lioz limestone, widely used in Portuguese colonial buildings and recognized as a heritage stone by the International Union of Geological Sciences. Found in the historic buildings of Manaus, (Brazil), this limestone was influenced by Portuguese architecture and adds social and cultural value to the city. Therefore, initiatives are needed to record and conserve Lioz limestone in historic buildings in Manaus, which could generate opportunities for geotourism and geoeducational practices. This paper presents a qualitative inventory of geodiversity and a virtual record of the fossil assemblage found in Lioz limestone in the main historic buildings of Manaus, Brazil. The use of Lioz limestone was categorized as structural, functional, or decorative, and the fossil assemblage was identified and represented by bivalves (<i>Caprinula</i> and <i>Radiolites</i>), gastropods (<i>Neoptyxis</i>), and trace fossils (<i>Thalassinoides</i>). An online geotourism itinerary featuring Lioz limestone uses was created and is supported by panoramic images and 3D models of six historical buildings. The itinerary aims to promote interdisciplinary integration and strengthen the geoconservation of stone heritage in the historic center of Manaus.</p>