<p>Utilizing high-quality monogerm sugar beet seeds is effective due to their consistent germination. Germination is influenced by environmental factors and plant hormones. To produce monogerm sugar beet seeds with uniform germination, ten single crosses were created from the mating of ten male sterile lines with a male parent across three locations in Iran: Ardabil, Firuzkuh, and Karaj. Consequently, three single cross progenies were selected based on seed quality traits for further analysis of their physical and chemical properties, as well as growth regulators. A combined analysis of variance revealed that the germination percentage of single crosses cultivated in Karaj, characterized by higher temperatures and lower humidity, was superior compared to those in Ardabil and Firuzkuh. Anion levels, particularly oxalate and phosphate, were significantly higher in the embryo and fruit pericarp of seeds with low sprouting rates, showing a notable positive correlation with germination duration. The electrical conductivity (EC) of seed extracts increased with the accumulation of ions in the fruit pericarp, although no significant correlation was found between phosphate levels and EC. Therefore, the accumulation of oxalate and insoluble phosphate exhibited significant positive correlations with seed thickness. Besides, ABA and IAA contents were accumulated in dry seeds of the single cross with higher germination rate, but it declined significantly after 55 and 65 h of imbibition. Fluctuations in the concentrations of ABA and IAA, along with the levels of oxalate, chloride, and phosphorus in the fruit pericarp, could be essential factors influencing the development of sugar beet radicles.</p>