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<p>Drought, salinity, and heat are abiotic stresses imposed by environmental factors, significantly reducing the productivity of sugarcane, a crucial crop used to produce sucrose and bioethanol worldwide. Due to its large and complex genome, traditional breeding strategies have not yet been able to solve these challenges. In this research, we have analysed how sugarcane stress tolerance mechanisms are influenced by phospholipases (PLPs), which are essential lipid signalling enzymes. To find the potential genes implicated in stress responses, a genome-wide investigation of the phospholipase gene family in <i>Saccharum</i> species, including <i>S. spontaneum</i>, <i>S. officinarum</i>, and <i>S</i>. <i>hybrids,</i> was conducted and identified 69 <i>ShPLPs</i>, 8 <i>SoPLPs</i>, and 10 <i>SsPLPs</i> genes. Parameters such as protein size, molecular weight, isoelectric point, and hydropathy index exhibited variation, indicating a change of functions in stress adaptation among the PLPs gene family. Conserved motif analysis revealed five motifs with varying frequency and different arrangements, indicating the changes in functional conservation and species-specific adaptations. Multiple sequence and motif analysis shows evolutionary conservation and divergence in all species, especially in <i>Saccharum</i> hybrids. Phylogenetic analysis of PLPs genes in <i>Saccharum</i> species (S. hybrids, <i>S. officinarum</i>, <i>S.spontaneum</i>) and related Poaceae crops reveals functional divergence, complex evolutionary relationships, and high sequence conservation reflecting hybrid origins and adaptations. These findings enhance the understanding of the role of PLPs gene in the sugarcane breeding program. Gene structure analysis shows that these genes possess relatively compact structures with potential alternative splicing. These findings suggest that PLPs genes play vital roles in the regulatory networks of sugarcane, lipid metabolism, and stress signalling, providing useful information for breeding new stress-resistant cultivars to increase production in the context of climate change.</p>

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In Silico Analysis of Phospholipases Involved in Drought Stress in Lipid Signalling: A Genome-Wide Study in Saccharum Species

  • 期刊论文

  • 发表日期：

    2025年11月25日

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  • Palanivel Gopal Santhosh Kumar,
  • Rachel Lissy Vargheese,
  • Nandini Karthikeyan,
  • Indusha Yazhini Sankararaj,
  • Akila Dharshini Venkatachalam,
  • Manimekalai Ramaswamy,
  • Lakshmi Kasirajan

摘要

Drought, salinity, and heat are abiotic stresses imposed by environmental factors, significantly reducing the productivity of sugarcane, a crucial crop used to produce sucrose and bioethanol worldwide. Due to its large and complex genome, traditional breeding strategies have not yet been able to solve these challenges. In this research, we have analysed how sugarcane stress tolerance mechanisms are influenced by phospholipases (PLPs), which are essential lipid signalling enzymes. To find the potential genes implicated in stress responses, a genome-wide investigation of the phospholipase gene family in Saccharum species, including S. spontaneum, S. officinarum, and S. hybrids, was conducted and identified 69 ShPLPs, 8 SoPLPs, and 10 SsPLPs genes. Parameters such as protein size, molecular weight, isoelectric point, and hydropathy index exhibited variation, indicating a change of functions in stress adaptation among the PLPs gene family. Conserved motif analysis revealed five motifs with varying frequency and different arrangements, indicating the changes in functional conservation and species-specific adaptations. Multiple sequence and motif analysis shows evolutionary conservation and divergence in all species, especially in Saccharum hybrids. Phylogenetic analysis of PLPs genes in Saccharum species (S. hybrids, S. officinarum, S.spontaneum) and related Poaceae crops reveals functional divergence, complex evolutionary relationships, and high sequence conservation reflecting hybrid origins and adaptations. These findings enhance the understanding of the role of PLPs gene in the sugarcane breeding program. Gene structure analysis shows that these genes possess relatively compact structures with potential alternative splicing. These findings suggest that PLPs genes play vital roles in the regulatory networks of sugarcane, lipid metabolism, and stress signalling, providing useful information for breeding new stress-resistant cultivars to increase production in the context of climate change.