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<p>In sugarcane, <i>Fusarium</i> spp. cause two distinct diseases: wilt, which affects the stalk, and pokkah boeng (PB), which affects the foliage. <i>F. sacchari</i> has been associated with wilt, while various <i>Fusarium</i> spp. have been linked to PB. Previous reports from India have indicated that the same <i>F. sacchari</i> isolate can cause both diseases. In this study, we assessed the diversity of <i>Fusarium</i> species associated with wilt and PB in sugarcane. A total of 85 <i>Fusarium</i> isolates associated with the two diseases were identified using Fusarium-specific primers Fs1 for the partial gene of translation elongation factor (Tef) 1-α. Among the 60 wilt&#xa0;isolates, 32 were identified as <i>F. sacchari</i> (<i>Fs</i>), 17 as <i>F. andiyazi</i> (<i>Fa</i>), seven as <i>F. verticillioides</i> (<i>Fv</i>), and one each as <i>F. ficicrescens</i> (<i>Ff</i>), <i>F. nygamai</i> (<i>Fn</i>), <i>F. thapsinum</i> (<i>Ft</i>), and <i>F. proliferatum</i> (<i>Fp</i>). Of 25&#xa0;PB isolates, 17 were <i>Fs</i>; four were each of <i>Fa</i> and <i>Fv</i>. Additionally, a cane sample with mixed infections of wilt and PB was identified as <i>F. thapsinum</i>. Overall, 49 were identified as <i>F. sacchari</i>, 21 as <i>Fa</i> and 11 as <i>Fv</i> and four as <i>Ff</i>, <i>Fp</i>, <i>Fn,</i> and <i>Ft</i>. Phylogenetic analysis revealed eight broad clades along with six independent ones. Similarly, the <i>Fusarium</i> isolates associated with PB were distributed across seven clades. The 68 Tamil Nadu isolates were distributed across the clades and identified as <i>Fs</i> (40)<i>, Fa</i> (14)<i>, Fv</i> (10)<i>,</i> and one each of <i>Ff, Fn, Fp,</i> and<i> Ft</i>. The study also revealed that the same isolate of the pathogen could cause both wilt and PB diseases at different locations on a single variety or across two varieties. This study provides new information on the diversity of wilt and pokkah boeng-associated Fusaria in sugarcane, and further studies will contribute to a better understanding on epidemiology of the diseases.</p>

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Molecular Mapping and Diversity of Fusarium Isolates Associated with Wilt and Pokkah Boeng of Sugarcane in Tropical India

  • 期刊论文

  • 发表日期：

    2025年8月11日

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  • R. Viswanathan,
  • R. Selvakumar,
  • Chandramani Raj,
  • R. Gopi,
  • P. Malathi,
  • A. Ramesh Sundar,
  • A. Annadurai,
  • S. Balasaravanan,
  • R. Nithyanantham,
  • K. Kaverinathan,
  • K. Manivannan

摘要

In sugarcane, Fusarium spp. cause two distinct diseases: wilt, which affects the stalk, and pokkah boeng (PB), which affects the foliage. F. sacchari has been associated with wilt, while various Fusarium spp. have been linked to PB. Previous reports from India have indicated that the same F. sacchari isolate can cause both diseases. In this study, we assessed the diversity of Fusarium species associated with wilt and PB in sugarcane. A total of 85 Fusarium isolates associated with the two diseases were identified using Fusarium-specific primers Fs1 for the partial gene of translation elongation factor (Tef) 1-α. Among the 60 wilt isolates, 32 were identified as F. sacchari (Fs), 17 as F. andiyazi (Fa), seven as F. verticillioides (Fv), and one each as F. ficicrescens (Ff), F. nygamai (Fn), F. thapsinum (Ft), and F. proliferatum (Fp). Of 25 PB isolates, 17 were Fs; four were each of Fa and Fv. Additionally, a cane sample with mixed infections of wilt and PB was identified as F. thapsinum. Overall, 49 were identified as F. sacchari, 21 as Fa and 11 as Fv and four as Ff, Fp, Fn, and Ft. Phylogenetic analysis revealed eight broad clades along with six independent ones. Similarly, the Fusarium isolates associated with PB were distributed across seven clades. The 68 Tamil Nadu isolates were distributed across the clades and identified as Fs (40), Fa (14), Fv (10), and one each of Ff, Fn, Fp, and Ft. The study also revealed that the same isolate of the pathogen could cause both wilt and PB diseases at different locations on a single variety or across two varieties. This study provides new information on the diversity of wilt and pokkah boeng-associated Fusaria in sugarcane, and further studies will contribute to a better understanding on epidemiology of the diseases.