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<p>Dextran, recognized as a deleterious component in sugar production, impairs the manufacturing process, thereby highlighting the need for an efficient detection method. The modified alcohol haze method, traditionally employed for dextran detection, is hindered by complex procedures and a lack of specificity. In contrast, immunonephelometry, a more contemporary method which detects antigen–antibody complexes via light scattering, offers the advantages of specificity, ease of operation, and rapid detection, yet it incurs higher cost due to the expensive production of monoclonal antibody. Addressing these limitations, we have developed a latex-enhanced turbidimetric immunoassay (LETIA) that significantly reduces assay costs while retaining the advantages of its predecessors. The antibody was purified cost-effectively using a hydroxyapatite column. After optimization, the preparation of immune latex showed high repeatability, with coefficients of variation (CV) between 2.12 and 3.58%. The method exhibited a robust linear response within the concentration range of 0–100&#xa0;mg/L, as evidenced by an <i>R</i><sup>2</sup> value of 0.9985. The limit of detection (LOD) was 35&#xa0;mg/kg sugar, which indicated an excellent sensitivity. The CV was between 1.51 and 3.14% in high- and low-level repeatability test. The recovery is between 93.8 and 105.8% in high- and low-level fortification experiments. Common interferents did not exhibit any significant interference. The antibody dosage and test cost were 0.00455&#xa0;mg/test and 0.41 $/test, respectively, equating to a mere 1.5% and 4.8% of the immunonephelometry requirements, representing a significant reduction.</p>

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Establishment and Methodology Validation of Latex-Enhanced Turbidimetric Immunoassay for Dextran Determination in Sugar

  • 期刊论文

  • 发表日期：

    2025年6月8日

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  • Guowei Chang,
  • Jun Liu,
  • Bin Feng,
  • Jun Chen,
  • Qing Huang,
  • Guiyun Liu,
  • Xiuyu Luo,
  • Zhide Li,
  • Zengwei Huang,
  • Dafeng Liang

摘要

Dextran, recognized as a deleterious component in sugar production, impairs the manufacturing process, thereby highlighting the need for an efficient detection method. The modified alcohol haze method, traditionally employed for dextran detection, is hindered by complex procedures and a lack of specificity. In contrast, immunonephelometry, a more contemporary method which detects antigen–antibody complexes via light scattering, offers the advantages of specificity, ease of operation, and rapid detection, yet it incurs higher cost due to the expensive production of monoclonal antibody. Addressing these limitations, we have developed a latex-enhanced turbidimetric immunoassay (LETIA) that significantly reduces assay costs while retaining the advantages of its predecessors. The antibody was purified cost-effectively using a hydroxyapatite column. After optimization, the preparation of immune latex showed high repeatability, with coefficients of variation (CV) between 2.12 and 3.58%. The method exhibited a robust linear response within the concentration range of 0–100 mg/L, as evidenced by an R2 value of 0.9985. The limit of detection (LOD) was 35 mg/kg sugar, which indicated an excellent sensitivity. The CV was between 1.51 and 3.14% in high- and low-level repeatability test. The recovery is between 93.8 and 105.8% in high- and low-level fortification experiments. Common interferents did not exhibit any significant interference. The antibody dosage and test cost were 0.00455 mg/test and 0.41 $/test, respectively, equating to a mere 1.5% and 4.8% of the immunonephelometry requirements, representing a significant reduction.