We investigate periodic orbits of \(C^1\) autonomous vector fields in \(\mathbb {R}^n\) using inverse Jacobi multipliers that may depend explicitly on time. We establish a localization principle for T-periodic orbits in arbitrary dimension, extending known planar results and deriving nonexistence conditions through the relation between the time-slices \(V(0, \cdot )\) and \(V(T, \cdot )\) . We further characterize hyperbolicity and orbital stability, including a decomposition of characteristic multipliers along invariant surfaces associated with autonomous inverse Jacobi multipliers. A test for the algebraicity of periodic orbits in 3-dimensional vector fields is given based on non-autonomous inverse Jacobi multipliers. The interplay between normalizers, inverse Jacobi multipliers and invariants is analyzed, with applications to the Lorenz and Rössler systems.