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<p>Late embryogenesis abundant (LEA) proteins are a group of proteins that are induced by various abiotic stresses and play critical roles in protecting plants from environmental damage. Here, a LEA_2 encoding gene (<i>LEA2-2</i>) isolated from <i>Caragana korshinskii</i> was proved to be induced by salt, drought, dehydration, high pH, cold, heat, and the phytohormone abscisic acid (ABA). Moreover, overexpression of <i>CkLEA2-2</i> in <i>Escherichia coli</i> improved tolerance to salt and osmotic stress. The <i>CkLEA2-2</i> overexpression (OE) lines showed higher seed germination rates and longer primary roots than those of wild-type <i>Arabidopsis</i> plants under salt stress. Similarly, seed germination rates of OE lines were also higher than those of wild-type when subjected to osmotic stress. In addition, the OE lines showed reduced ABA sensitivity during seed germination and root growth compared with the wild-type. Several ABA- and stress-responsive marker genes were verified to be up-regulated in the OE lines too. Taken together, our results showed that CkLEA2-2 was a positive regulator involved in plant response to salt stress.</p>

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LEA2-2 from Caragana korshinskii confers salt and osmotic tolerance in Arabidopsis

  • 期刊论文

  • 发表日期：

    2026年7月8日

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  • Xiumin Yu,
  • Wenran Yue,
  • Qi Yang,
  • Chunmei Xue,
  • Fengyan Yi,
  • Shuan Guo,
  • Guojing Li

摘要

Late embryogenesis abundant (LEA) proteins are a group of proteins that are induced by various abiotic stresses and play critical roles in protecting plants from environmental damage. Here, a LEA_2 encoding gene (LEA2-2) isolated from Caragana korshinskii was proved to be induced by salt, drought, dehydration, high pH, cold, heat, and the phytohormone abscisic acid (ABA). Moreover, overexpression of CkLEA2-2 in Escherichia coli improved tolerance to salt and osmotic stress. The CkLEA2-2 overexpression (OE) lines showed higher seed germination rates and longer primary roots than those of wild-type Arabidopsis plants under salt stress. Similarly, seed germination rates of OE lines were also higher than those of wild-type when subjected to osmotic stress. In addition, the OE lines showed reduced ABA sensitivity during seed germination and root growth compared with the wild-type. Several ABA- and stress-responsive marker genes were verified to be up-regulated in the OE lines too. Taken together, our results showed that CkLEA2-2 was a positive regulator involved in plant response to salt stress.