ABA treatment on AtAVT6D-overexpressing Arabidopsis thaliana plants results in perturbation of ROS pathway genes
摘要
The present work is performed on AtAVT6D-overexpressor and wild-type Arabidopsis thaliana plants. The objective of the study is to examine changes in the gene expression profile after treatment with abscisic acid for 12 h and 24 h using a transcriptomics approach. 24 h-ABA-treated AtAVT6D overexpressors had the highest number of differentially expressed genes [9847] in response to stress compared to wild-type plants. Many of these genes in AtAVT6D overexpressors were involved in ROS scavenging pathways. Gene-ontological analysis of 24 h-ABA-treated plants indicated an increased response to oxygen. The upregulated genes in wild-type plants were related to energy generation pathways. KEGG analysis depicted ‘Carbon metabolism’, ‘Amino acid metabolism’, and ‘Co-factor biosynthesis’ as the enriched pathways at 12 h and 24 h ABA treatment conditions. RNA-sequencing results were validated by RT-qPCR analysis of few candidate genes. The findings in this article are consistent with our previous study on AtAVT6D gene and further demonstrate that AtAVT6D overexpression broadly reprograms the transcriptome in response to ABA, particularly affecting ROS-related pathways. This work will help us understand the role of AtAVT6D gene in detail under stress response.