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<p>Calcium-dependent protein kinase (CDPK) is a type of Ca<sup>2+</sup>- sensitive Ser/Thr protein kinase that plays a role in plant growth, development, and response to stress. However, the function of the <i>CDPK</i> gene family in sugar beet (<i>Beta vulgaris</i> L.) is still unknown. In this study, a total of 16 <i>BvCDPK</i> genes in sugar beet were firstly identified by bioinformatics techniques, and they were unevenly distributed across seven chromosomes. The <i>BvCDPK</i> genes were divided into four clusters (I–IV) by phylogenetic analysis, and members of the same family were found to have similar protein motifs and gene structures. Expression profiling under salt stress via qRT-PCR showed that most <i>BvCDPKs</i> were up-regulated, with <i>BvCDPK8</i> consistently induced in both roots and leaves. Transgenic <i>Arabidopsis thaliana</i> plants overexpressing <i>BvCDPK8</i> exhibited significantly improved growth under salt stress, accompanied by increased levels of proline, soluble sugars, and total chlorophyll, enhanced activities of antioxidant enzymes such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and marked reduction in malondialdehyde (MDA) content. Furthermore, the expression levels of stress-responsive genes (<i>AtNCED3</i>, <i>AtSOS1</i>, and <i>AtP5CS</i>) were significantly up-regulated in transgenic plants, with <i>AtNCED3</i> expression in the OE4 line being 3.05-fold higher than in wild-type (WT) plants under 100 mM NaCl. Together, these results demonstrated that <i>BvCDPK8</i> confers enhanced salt tolerance in <i>A. thaliana</i>, providing a solid foundation for further investigation into its molecular mechanisms.</p>

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Identification of the BvCDPK gene family in sugar beet (Beta vulgaris L.) at a genome-wide level and analysis of the function of BvCDPK8 under salt conditions

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Yan Nan,
  • Min Li,
  • Bin Cheng,
  • Ming Wei,
  • Guo-Qiang Wu

摘要

Calcium-dependent protein kinase (CDPK) is a type of Ca2+- sensitive Ser/Thr protein kinase that plays a role in plant growth, development, and response to stress. However, the function of the CDPK gene family in sugar beet (Beta vulgaris L.) is still unknown. In this study, a total of 16 BvCDPK genes in sugar beet were firstly identified by bioinformatics techniques, and they were unevenly distributed across seven chromosomes. The BvCDPK genes were divided into four clusters (I–IV) by phylogenetic analysis, and members of the same family were found to have similar protein motifs and gene structures. Expression profiling under salt stress via qRT-PCR showed that most BvCDPKs were up-regulated, with BvCDPK8 consistently induced in both roots and leaves. Transgenic Arabidopsis thaliana plants overexpressing BvCDPK8 exhibited significantly improved growth under salt stress, accompanied by increased levels of proline, soluble sugars, and total chlorophyll, enhanced activities of antioxidant enzymes such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and marked reduction in malondialdehyde (MDA) content. Furthermore, the expression levels of stress-responsive genes (AtNCED3, AtSOS1, and AtP5CS) were significantly up-regulated in transgenic plants, with AtNCED3 expression in the OE4 line being 3.05-fold higher than in wild-type (WT) plants under 100 mM NaCl. Together, these results demonstrated that BvCDPK8 confers enhanced salt tolerance in A. thaliana, providing a solid foundation for further investigation into its molecular mechanisms.