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<p>Sesquiterpenoids are one of the secondary metabolites in agarwood which, is a profitable traditional Chinese medicine and spice. Agarwood is derived from the injured <i>Aquilaria sinensis</i>. <i>Chi-Nan</i> germplasm is a new chemotype of <i>A. sinensis</i> that exhibits a higher capacity of agarwood production and the diversity of sesquiterpenoid biosynthesis. To examine the mechanism of sesquiterpene generation from <i>Chi-Nan</i> germplasm<i>,</i> a 1812&#xa0;bp-length <i>sesquiterpene synthase 15</i> (<i>AsSS15</i>) gene encoding 603 amino acids was obtained from the wounded branches. <i>AsSS15</i> showed significant expression levels in the stem and root that the major tissues of agarwood production, indicating that it was involved in agarwood formation. <i>AsSS15</i> expression was higher in wounded branches of <i>Chi-Nan</i> germplasms than that in ordinary germplasms of <i>A. sinensis</i>. The AsSS15 recombinant protein was successfully expressed, and catalyzed farnesyl pyrophosphate (FPP) substrates to generate nerolidol in vitro. These findings demonstrated that <i>AsSS15</i> significantly induces the expression of gene related to sesquiterpene biosynthesis in <i>Chi-Nan</i> germplasm. This study first elucidates potential mechanisms of sesquiterpene generation from <i>Chi-Nan</i> germplasms, which adds to the theory of sesquiterpene accumulation in <i>A. sinensis</i> and serves as a foundation for future research into the biosynthesis and application of <i>Chi-Nan</i> germplasm.</p>

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Molecular cloning, prokaryotic expression and induction characteristics of the sesquiterpene synthase gene (AsSS15) from the Chi-Nan germplasm (Aquilaria sinensis)

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  • 出版模式：

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  • 发表日期：

    2025年9月4日

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  • Zhenghan Bao,
  • Peiwen Sun,
  • Jianhe Wei,
  • Xiaohong Fao,
  • Feifei Lv,
  • Yun Yang

摘要

Sesquiterpenoids are one of the secondary metabolites in agarwood which, is a profitable traditional Chinese medicine and spice. Agarwood is derived from the injured Aquilaria sinensis. Chi-Nan germplasm is a new chemotype of A. sinensis that exhibits a higher capacity of agarwood production and the diversity of sesquiterpenoid biosynthesis. To examine the mechanism of sesquiterpene generation from Chi-Nan germplasm, a 1812 bp-length sesquiterpene synthase 15 (AsSS15) gene encoding 603 amino acids was obtained from the wounded branches. AsSS15 showed significant expression levels in the stem and root that the major tissues of agarwood production, indicating that it was involved in agarwood formation. AsSS15 expression was higher in wounded branches of Chi-Nan germplasms than that in ordinary germplasms of A. sinensis. The AsSS15 recombinant protein was successfully expressed, and catalyzed farnesyl pyrophosphate (FPP) substrates to generate nerolidol in vitro. These findings demonstrated that AsSS15 significantly induces the expression of gene related to sesquiterpene biosynthesis in Chi-Nan germplasm. This study first elucidates potential mechanisms of sesquiterpene generation from Chi-Nan germplasms, which adds to the theory of sesquiterpene accumulation in A. sinensis and serves as a foundation for future research into the biosynthesis and application of Chi-Nan germplasm.