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<p>There are multiple studies exploring the role of circulatory micro RNAs (miRNAs) in prediabetes. However there were no studies addressing the circulatory miRNAs dysregulated in isolated impaired fasting glycemia (iIFG) patients when compared to healthy subjects and type 2 diabetes mellitus (T2DM) patients. Nine pooled samples each from iIFG, normal glucose tolerance (NGT) and T2DM were used for profiling of 89 circulatory miRNAs using Qiagen Custom miRCURY LNA miRNA polymerase chain reaction (PCR) panels. Top five upregulated miRNAs; 15b-5p, miR-21-5p, miR-27b-3p, miR-142-3p, and miR-144-3p were validated on 50 NGT, 41 iIFG and 42 T2DM patients using qRT-PCR. Receiver operating characteristic (ROC) curve analysis was performed to assess their diagnostic utility. A total of 26 miRNAs were differentially expressed in iIFG group in comparison to controls and 14 miRNAs differentially expressed in iIFG group compared to T2DM group. Validation experiments showed that miR-15b-5p, miR-21-5p, miR-27b-3p, and miR-142-3p were consistently overexpressed in plasma samples of iIFG patients compared to healthy controls. In ROC curve analysis, miR-15b-5p, miR-27b-3p, and miR-142-3p displayed excellent specificity and sensitivity with area under the curve of 0.838, 0.839, and 0.896. Present study showed the differential expression of a set of miRNAs in iIFG group in comparison to controls. Out of them miR-15b-5p, miR-27b-3p, and miR-142-3p showed a very good diagnostic utility.</p>

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Plasma MicroRNA Expression Differentiates Individuals with Isolated Impaired Fasting Glycaemia from Normal Glucose Tolerance and Type 2 Diabetes Mellitus

  • 期刊论文

  • 发表日期：

    2026年2月7日

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  • Palaniswamy Ramaswamy,
  • Pratibha Misra,
  • Swati Bajaj,
  • G. Ankita,
  • G. Ruchira,
  • P. Arathi,
  • Bhasker Mukherjee,
  • Anurodh Gupta,
  • Y. Vashum,
  • Amrita Parmar,
  • M. K. Sibin

摘要

There are multiple studies exploring the role of circulatory micro RNAs (miRNAs) in prediabetes. However there were no studies addressing the circulatory miRNAs dysregulated in isolated impaired fasting glycemia (iIFG) patients when compared to healthy subjects and type 2 diabetes mellitus (T2DM) patients. Nine pooled samples each from iIFG, normal glucose tolerance (NGT) and T2DM were used for profiling of 89 circulatory miRNAs using Qiagen Custom miRCURY LNA miRNA polymerase chain reaction (PCR) panels. Top five upregulated miRNAs; 15b-5p, miR-21-5p, miR-27b-3p, miR-142-3p, and miR-144-3p were validated on 50 NGT, 41 iIFG and 42 T2DM patients using qRT-PCR. Receiver operating characteristic (ROC) curve analysis was performed to assess their diagnostic utility. A total of 26 miRNAs were differentially expressed in iIFG group in comparison to controls and 14 miRNAs differentially expressed in iIFG group compared to T2DM group. Validation experiments showed that miR-15b-5p, miR-21-5p, miR-27b-3p, and miR-142-3p were consistently overexpressed in plasma samples of iIFG patients compared to healthy controls. In ROC curve analysis, miR-15b-5p, miR-27b-3p, and miR-142-3p displayed excellent specificity and sensitivity with area under the curve of 0.838, 0.839, and 0.896. Present study showed the differential expression of a set of miRNAs in iIFG group in comparison to controls. Out of them miR-15b-5p, miR-27b-3p, and miR-142-3p showed a very good diagnostic utility.