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<p>Recent epidemiological studies have highlighted the inverse relationship between high-density lipoprotein cholesterol (HDL-C) levels and coronary artery disease (CAD) risk. However, randomized control trials and Mendelian randomization studies have challenged the direct causative link between HDL-C levels and CAD incidence. The study aimed to evaluate two cell-based assays—THP-1 and J774 cells—and a liposome-based method in a cohort comprising 54 CAD patients and 34 healthy controls. Logistic regression and correlation analyses were employed to compare CEC results obtained from cell-based and liposome-based assays. The study findings showed a significant difference in CEC between CAD and control groups using cell-based assays (THP-1 and J774), while the liposome-based method did not distinguish between the groups. In conclusion, while liposome-based assays offer simplicity and feasibility for high-throughput screening, this method lack the sensitivity to accurately assess HDL function in the context of cardiovascular risk assessment compared to cell-based assays. Standardizing CEC protocols will be crucial for advancing our understanding of HDL biology and improving its clinical utility in predicting and managing cardiovascular disease effectively.</p>

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Comparison of HDL Cholesterol Efflux Capacity Assays and the Effect of Assay Selection on CEC and Coronary Heart Disease

  • 期刊论文

  • 发表日期：

    2025年2月5日

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  • Atanu Sen,
  • Himani Thakkar,
  • Vinnyfred Vincent,
  • Swati Varshney,
  • Archna Singh

摘要

Recent epidemiological studies have highlighted the inverse relationship between high-density lipoprotein cholesterol (HDL-C) levels and coronary artery disease (CAD) risk. However, randomized control trials and Mendelian randomization studies have challenged the direct causative link between HDL-C levels and CAD incidence. The study aimed to evaluate two cell-based assays—THP-1 and J774 cells—and a liposome-based method in a cohort comprising 54 CAD patients and 34 healthy controls. Logistic regression and correlation analyses were employed to compare CEC results obtained from cell-based and liposome-based assays. The study findings showed a significant difference in CEC between CAD and control groups using cell-based assays (THP-1 and J774), while the liposome-based method did not distinguish between the groups. In conclusion, while liposome-based assays offer simplicity and feasibility for high-throughput screening, this method lack the sensitivity to accurately assess HDL function in the context of cardiovascular risk assessment compared to cell-based assays. Standardizing CEC protocols will be crucial for advancing our understanding of HDL biology and improving its clinical utility in predicting and managing cardiovascular disease effectively.