Scholarlink

<p>Living biological systems sense metabolic cues and translate them into functional responses. Reconstructing these capabilities in bottom-up synthetic biology enables the design of artificial systems with life-like regulation. Here, we describe a phase separation-based synthetic organelle that couples a metabolic signal to transcriptional control. By engineering a pyruvate-responsive DNA-binding protein to undergo liquid-liquid phase separation, we created membrane-less compartments that reversibly sequester DNA and thereby modulate gene transcription in a dose-dependent manner. These results establish metabolite-responsive synthetic organelles as versatile building blocks for synthetic cells and responsive biomaterials.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metaboliten-abhängige Genexpression in synthetischen Organellen

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月18日

查看全文
  • Carolina Jerez-Longres,
  • Wilfried Weber

摘要

Living biological systems sense metabolic cues and translate them into functional responses. Reconstructing these capabilities in bottom-up synthetic biology enables the design of artificial systems with life-like regulation. Here, we describe a phase separation-based synthetic organelle that couples a metabolic signal to transcriptional control. By engineering a pyruvate-responsive DNA-binding protein to undergo liquid-liquid phase separation, we created membrane-less compartments that reversibly sequester DNA and thereby modulate gene transcription in a dose-dependent manner. These results establish metabolite-responsive synthetic organelles as versatile building blocks for synthetic cells and responsive biomaterials.