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<p>Endocytic and retrograde trafficking of transmembrane proteins from the plasma membrane to endolysosomal compartments and the trans-Golgi network is crucial for membrane homeostasis and cellular signaling. We developed a versatile toolkit of bacterially expressed, functionalized anti-GFP nanobodies that enables biochemical, live-cell, and electron microscopy tracking of endocytosed proteins, allowing systematic analysis of their intracellular fate and sorting routes.</p>

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Nano-GPS-Tracking der Endozytose

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月25日

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  • Dominik P. Buser

摘要

Endocytic and retrograde trafficking of transmembrane proteins from the plasma membrane to endolysosomal compartments and the trans-Golgi network is crucial for membrane homeostasis and cellular signaling. We developed a versatile toolkit of bacterially expressed, functionalized anti-GFP nanobodies that enables biochemical, live-cell, and electron microscopy tracking of endocytosed proteins, allowing systematic analysis of their intracellular fate and sorting routes.