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<p>We introduce a Fluorescence Integrated Single-Cell Analysis Script (FISCAS) that combines fluorescence microscopy with MALDI-MSI to streamline single-cell analysis. FISCAS enables automated selection of tight measurement regions, thereby reducing the acquisition of off-target pixels, and makes use of established algorithms for cell segmentation and coregistration to rapidly compile single-cell mass spectra.</p>

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FISCAS — ein Werkzeug zur effizienten Erzeugung von Einzelzellspektren

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月4日

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  • Jan Schwenzfeier,
  • Jens Soltwisch

摘要

We introduce a Fluorescence Integrated Single-Cell Analysis Script (FISCAS) that combines fluorescence microscopy with MALDI-MSI to streamline single-cell analysis. FISCAS enables automated selection of tight measurement regions, thereby reducing the acquisition of off-target pixels, and makes use of established algorithms for cell segmentation and coregistration to rapidly compile single-cell mass spectra.