Scholarlink

<p>This study aimed to investigate the anti-inflammatory effects of <i>Tetradesmus wisconsinensis</i> extract (TWE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. To evaluate TWE’s inflammation-modulating activity, we analyzed its secondary metabolite content and antioxidant capacity. The extract contained 1.98&#xa0;mg gallic acid equivalents/g dry matter of total phenolic content and 0.5&#xa0;mg quercetin equivalents/g dry matter of total flavonoid content and also exhibited 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging activities of 9% and 20.1%, respectively, indicating a contributory antioxidant capacity associated with its secondary metabolite composition. High-performance liquid chromatography analysis identified lutein (1.6&#xa0;mg/g dry extract) as the major constituent, indicating that carotenoid accumulation may partially contribute to the observed bioactivities. Following cell viability assessment, flow cytometric analysis showed that TWE markedly reduced LPS-induced macrophage activation-associated responses, with an inhibition rate of up to 80.5%. This reduction was accompanied by a significant inhibition of nitric oxide (NO) production, with TWE and lutein reducing NO levels by 42.4% and 28.6%, respectively, compared with the LPS control. Consistent with these findings, TWE significantly downregulated the expression of inflammatory genes (up to 37.8%) and proteins (up to 55.4% and 17.9–48.9% for TWE and lutein, respectively), suggesting the involvement of the nuclear factor-kappa B (NF-κB) signaling pathway. Immunofluorescence analysis further revealed 42–76.3% reductions in fluorescence intensity, supporting the modulation of NF-κB-related inflammatory signaling and attenuation of p65 nuclear translocation. Taken together, these results demonstrate that TWE exhibits significant in vitro anti-inflammatory activity in macrophages, and support its potential as a bioactive ingredient.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Anti-inflammatory effects of Tetradesmus wisconsinensis extract and lutein in lipopolysaccharide-stimulated RAW 264.7 macrophages via nuclear factor-kappa B-related signaling

  • 期刊论文

  • 发表日期：

    2026年6月26日

查看全文
  • Do Hyeon Jin,
  • Min Ho Han,
  • Jong Hyun Oh,
  • Ju Hwan Lee,
  • Jin Woo Kim

摘要

This study aimed to investigate the anti-inflammatory effects of Tetradesmus wisconsinensis extract (TWE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. To evaluate TWE’s inflammation-modulating activity, we analyzed its secondary metabolite content and antioxidant capacity. The extract contained 1.98 mg gallic acid equivalents/g dry matter of total phenolic content and 0.5 mg quercetin equivalents/g dry matter of total flavonoid content and also exhibited 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging activities of 9% and 20.1%, respectively, indicating a contributory antioxidant capacity associated with its secondary metabolite composition. High-performance liquid chromatography analysis identified lutein (1.6 mg/g dry extract) as the major constituent, indicating that carotenoid accumulation may partially contribute to the observed bioactivities. Following cell viability assessment, flow cytometric analysis showed that TWE markedly reduced LPS-induced macrophage activation-associated responses, with an inhibition rate of up to 80.5%. This reduction was accompanied by a significant inhibition of nitric oxide (NO) production, with TWE and lutein reducing NO levels by 42.4% and 28.6%, respectively, compared with the LPS control. Consistent with these findings, TWE significantly downregulated the expression of inflammatory genes (up to 37.8%) and proteins (up to 55.4% and 17.9–48.9% for TWE and lutein, respectively), suggesting the involvement of the nuclear factor-kappa B (NF-κB) signaling pathway. Immunofluorescence analysis further revealed 42–76.3% reductions in fluorescence intensity, supporting the modulation of NF-κB-related inflammatory signaling and attenuation of p65 nuclear translocation. Taken together, these results demonstrate that TWE exhibits significant in vitro anti-inflammatory activity in macrophages, and support its potential as a bioactive ingredient.