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<p>Glycerol represents a promising feedstock for sustainable bioconversion into high-value chemicals, available either as surplus crude glycerol from biodiesel production or through biosynthesis from glucose depending on market conditions. However, the industrial host <i>Corynebacterium glutamicum</i> lacks the native ability to utilize glycerol as a sole carbon source, limiting its application in glycerol-based bioprocesses. In this study, we engineered <i>C. glutamicum</i> by introducing two novel heterologous pathways from <i>Bacillus licheniformis</i>: a glycerol utilization pathway and a glycerol biosynthesis pathway. The engineered strain harboring the glycerol utilization pathway (<i>glpFKD</i> genes) exhibited significantly faster glycerol consumption, 1.6-fold and 3.0-fold increase, compared to that of the strains utilizing conventional pathways from <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i>, respectively. Additionally, by integrating the best 1,3-propanediol (1,3-PDO) synthesis module (<i>yqhD</i>,<i> dhaB-gdrAB</i> genes), we achieved 27.8&#xa0;g/L of 1,3-PDO from glycerol in flask cultivation. Separately, the introduction and optimization of glycerol synthesis pathway (<i>g1pD-dgp</i> genes) enabled <i>C. glutamicum</i> to produce 10.1&#xa0;g/L of glycerol from glucose. Followed by additional introduction of the 1,3-PDO synthesis pathway, the final engineered strain could produce 4.7&#xa0;g/L of 1,3-PDO from glucose in flask cultivation. This work represents the first demonstration of functional implementation of both glycerol utilization and synthesis pathways from <i>B. licheniformis</i> in <i>C. glutamicum</i> and their applications toward 1,3-PDO production. These findings provide a versatile platform for the development of <i>C. glutamicum</i> as a host for the sustainable production of glycerol-derived biochemicals.</p>

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Introduction of novel glycerol utilization or synthesis pathway from Bacillus licheniformis for production of 1,3-propanediol in Corynebacterium glutamicum

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Je Woong Kim,
  • Chan Woo Song,
  • Jyotiranjan Bal,
  • Mina Kwon,
  • Sung Uk Kang,
  • Jong Myoung Park,
  • Hyohak Song

摘要

Glycerol represents a promising feedstock for sustainable bioconversion into high-value chemicals, available either as surplus crude glycerol from biodiesel production or through biosynthesis from glucose depending on market conditions. However, the industrial host Corynebacterium glutamicum lacks the native ability to utilize glycerol as a sole carbon source, limiting its application in glycerol-based bioprocesses. In this study, we engineered C. glutamicum by introducing two novel heterologous pathways from Bacillus licheniformis: a glycerol utilization pathway and a glycerol biosynthesis pathway. The engineered strain harboring the glycerol utilization pathway (glpFKD genes) exhibited significantly faster glycerol consumption, 1.6-fold and 3.0-fold increase, compared to that of the strains utilizing conventional pathways from Escherichia coli and Klebsiella pneumoniae, respectively. Additionally, by integrating the best 1,3-propanediol (1,3-PDO) synthesis module (yqhD, dhaB-gdrAB genes), we achieved 27.8 g/L of 1,3-PDO from glycerol in flask cultivation. Separately, the introduction and optimization of glycerol synthesis pathway (g1pD-dgp genes) enabled C. glutamicum to produce 10.1 g/L of glycerol from glucose. Followed by additional introduction of the 1,3-PDO synthesis pathway, the final engineered strain could produce 4.7 g/L of 1,3-PDO from glucose in flask cultivation. This work represents the first demonstration of functional implementation of both glycerol utilization and synthesis pathways from B. licheniformis in C. glutamicum and their applications toward 1,3-PDO production. These findings provide a versatile platform for the development of C. glutamicum as a host for the sustainable production of glycerol-derived biochemicals.