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<p><i>Calystegia soldanella</i> (L.) <i>Roem &amp; Schult</i> is a halophyte that adapts to harsh environments such as high light intensity and salinity and is known for its antipyretic, antibacterial, antioxidant, antiviral, and antifungal properties. This study aims to develop a bioactive with antioxidant and moisturizing effects from the cultivated adventitious root tissue of <i>Calystegia soldanella</i> (L.) <i>Roem &amp; Schult</i>. The hot water extract demonstrated no phototoxicity in the 3T3 NRU phototoxicity test. In a 3D artificial skin model composed of HaCaT and fibroblast co-cultures, the hot water extract at a concentration of 200&#xa0;μg/mL exhibited antioxidant effects superior to 50&#xa0;μM ascorbic acid and showed an increase in hyaluronic acid synthesis comparable to that of 50&#xa0;μM retinoic acid. mRNA analysis of HaCaT cells treated with the extract revealed the upregulation of HO-1, an antioxidant-related factor, and HAS2, associated with hyaluronic acid synthesis. Protein analysis of the 3D skin model confirmed the upregulation of HO-1 and its transcription factor p-Nrf2, as well as HAS2 and its transcription factors STAT3 and CREB. Additionally, HPLC analysis identified the presence of antioxidants such as skimmianine and 3,5-dicaffeoylquinic acid in the hot water extract. These findings suggest that the root tissue of <i>Calystegia soldanella</i> (L.) <i>Roem &amp; Schult</i>, obtained through root culture, exhibits antioxidant and moisturizing properties, highlighting its potential as a raw material for cosmetics or pharmaceuticals.</p>

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Identification of antioxidative and moisturizing efficacies of Calystegia soldanella (L.) Roem & Schult adventitious root cultivated in a controlled bioreactor using a 3D artificial skin substitute

  • 期刊论文

  • 发表日期：

    2025年8月6日

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  • Bum Jun Jang,
  • Jae Ahn Shin,
  • Jun Hyuk Oh,
  • Jong-Du Lee,
  • Seon-A Yoon,
  • Young Min Ham,
  • Jeong Mi Kim,
  • Hwa Sung Shin

摘要

Calystegia soldanella (L.) Roem & Schult is a halophyte that adapts to harsh environments such as high light intensity and salinity and is known for its antipyretic, antibacterial, antioxidant, antiviral, and antifungal properties. This study aims to develop a bioactive with antioxidant and moisturizing effects from the cultivated adventitious root tissue of Calystegia soldanella (L.) Roem & Schult. The hot water extract demonstrated no phototoxicity in the 3T3 NRU phototoxicity test. In a 3D artificial skin model composed of HaCaT and fibroblast co-cultures, the hot water extract at a concentration of 200 μg/mL exhibited antioxidant effects superior to 50 μM ascorbic acid and showed an increase in hyaluronic acid synthesis comparable to that of 50 μM retinoic acid. mRNA analysis of HaCaT cells treated with the extract revealed the upregulation of HO-1, an antioxidant-related factor, and HAS2, associated with hyaluronic acid synthesis. Protein analysis of the 3D skin model confirmed the upregulation of HO-1 and its transcription factor p-Nrf2, as well as HAS2 and its transcription factors STAT3 and CREB. Additionally, HPLC analysis identified the presence of antioxidants such as skimmianine and 3,5-dicaffeoylquinic acid in the hot water extract. These findings suggest that the root tissue of Calystegia soldanella (L.) Roem & Schult, obtained through root culture, exhibits antioxidant and moisturizing properties, highlighting its potential as a raw material for cosmetics or pharmaceuticals.