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<p>Sustainable alternatives to conventional plastics are needed because of increasing environmental challenges and the finite nature of fossil fuel resources. Poly(butylene adipate-<i>co</i>-terephthalate) (PBAT) has emerged as an alternative to conventional plastics owing to its excellent properties and good biodegradability. However, PBAT has a relatively low yield of degradation compared to other bioplastics, and monomers remain after degradation. To enhance PBAT degradation yields and effectively metabolize the resulting aliphatic monomers, a gene discovered through whole-genome sequencing of <i>Bacillus</i> sp. JY35 was introduced into <i>Pseudomonas putida</i> KT2440 to overexpress triacylglycerol lipase with high degradation activity. In the clear zone test, engineered <i>P. putida</i> KT2440 showed high degradation potential for various bioplastics, including PBAT, compared to other <i>P. putida</i> strains. In addition, for all PBAT monomers, engineered <i>P. putida</i> KT2440 showed higher consumption than the wild type, especially adipic acid and 1,4-butanediol. Specific activity against esterase was measured using <i>p</i>-nitrophenyl substrates, and the specific activity was higher than that of the wild type. Further quantitative analysis revealed that PBAT was degraded by 52.2% on day 21. The decreasing molecular weight and the surface changes resulting in cracks as degradation progressed were analyzed using gel permeation chromatography and scanning electron microscopy, respectively. We successfully overexpressed triacylglycerol lipase, an excellent bioplastic degradation enzyme, using <i>P. putida</i> KT2440, increasing the PBAT degradation yield and enabling effective aliphatic monomer consumption.</p>

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Construction of Pseudomonas putida KT2440 degrading poly(butylene adipate-co-terephthalate) and enhanced consumption of adipic acid and 1,4-butanediol

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Nara Shin,
  • Jinok Oh,
  • Gaeun Lim,
  • Yebin Han,
  • Yunhee Jeong,
  • Hee Taek Kim,
  • Jeong Chan Joo,
  • Sang Ho Lee,
  • Shashi Kant Bhatia,
  • Yung-Hun Yang

摘要

Sustainable alternatives to conventional plastics are needed because of increasing environmental challenges and the finite nature of fossil fuel resources. Poly(butylene adipate-co-terephthalate) (PBAT) has emerged as an alternative to conventional plastics owing to its excellent properties and good biodegradability. However, PBAT has a relatively low yield of degradation compared to other bioplastics, and monomers remain after degradation. To enhance PBAT degradation yields and effectively metabolize the resulting aliphatic monomers, a gene discovered through whole-genome sequencing of Bacillus sp. JY35 was introduced into Pseudomonas putida KT2440 to overexpress triacylglycerol lipase with high degradation activity. In the clear zone test, engineered P. putida KT2440 showed high degradation potential for various bioplastics, including PBAT, compared to other P. putida strains. In addition, for all PBAT monomers, engineered P. putida KT2440 showed higher consumption than the wild type, especially adipic acid and 1,4-butanediol. Specific activity against esterase was measured using p-nitrophenyl substrates, and the specific activity was higher than that of the wild type. Further quantitative analysis revealed that PBAT was degraded by 52.2% on day 21. The decreasing molecular weight and the surface changes resulting in cracks as degradation progressed were analyzed using gel permeation chromatography and scanning electron microscopy, respectively. We successfully overexpressed triacylglycerol lipase, an excellent bioplastic degradation enzyme, using P. putida KT2440, increasing the PBAT degradation yield and enabling effective aliphatic monomer consumption.