<p>Diabetes mellitus, a global health concern, is characterized by chronic hyperglycemia, which leads to severe complications and imposes substantial economic and healthcare burdens. Elevated blood glucose levels induce cellular senescence, impairing wound healing and contributing to chronic wounds in diabetic patients. This study investigates the potential of balloon flower root (<i>Platycodon grandiflorus</i>)-derived nanovesicles (BFR-NVs), known for their potent antioxidant properties, to mitigate hyperglycemia-induced impairments in wound healing. BFR-NVs were isolated using a hybrid method combining polyethylene glycol-based precipitation and ultracentrifugation, yielding vesicles with stable size distribution over two weeks of refrigerated storage. In vitro, treatment of high-glucose-induced senescent human dermal fibroblasts with BFR-NVs resulted in reduced expression of senescence-associated markers, normalization of gene expression profiles, and enhanced cellular proliferation and migration. These findings indicate the potential therapeutic role of BFR-NVs in mitigating hyperglycemia-induced senescence-associated cellular dysfunction. The antioxidant properties of BFR-NVs may play a crucial role in restoring cellular function, providing valuable insights for future research and clinical applications aimed at improving wound healing in diabetic patients.</p> Graphical abstract <p></p>