Scholarlink

<p>In this study, we develop a one-step microfluidic system to continuously generate cell spheroids for circadian rhythm analysis. We use a glass capillary device to encapsulate cells within water-fluorocarbon-water double emulsions, facilitating rapid spheroid formation through cell–cell interactions in a biocompatible environment. By adjusting the flow rate, we control the size of the emulsion droplets, effectively regulating the number of encapsulated cells and enabling precise control of spheroid size. Increasing the viscosity of the cell suspension prevents cell sedimentation, ensuring uniform cell encapsulation and spheroid formation. In the jetting regime, higher inertia allows for more consistent cell encapsulation compared to the dripping regime. Using the generated spheroids, we achieve more accurate circadian rhythm observations, revealing differences from two-dimensional culture results.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

One-step spheroid formation via double-emulsion drops generated by a glass capillary-based microfluidic device for circadian rhythm investigation

  • 期刊论文

  • 发表日期：

    2025年2月15日

查看全文
  • Hye-Seon Jeong,
  • Chae-Hee Park,
  • Chang-Soo Lee,
  • Kyung-Ha Lee,
  • Chang-Hyung Choi

摘要

In this study, we develop a one-step microfluidic system to continuously generate cell spheroids for circadian rhythm analysis. We use a glass capillary device to encapsulate cells within water-fluorocarbon-water double emulsions, facilitating rapid spheroid formation through cell–cell interactions in a biocompatible environment. By adjusting the flow rate, we control the size of the emulsion droplets, effectively regulating the number of encapsulated cells and enabling precise control of spheroid size. Increasing the viscosity of the cell suspension prevents cell sedimentation, ensuring uniform cell encapsulation and spheroid formation. In the jetting regime, higher inertia allows for more consistent cell encapsulation compared to the dripping regime. Using the generated spheroids, we achieve more accurate circadian rhythm observations, revealing differences from two-dimensional culture results.