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Purpose <p>The current study was conducted to assess the qualitative phytochemical profile, antioxidant, and antidiabetic effect of methanolic extract of GWDS (MEGWDS).</p> Methods <p>Phytochemical constituents, total phenolic, and flavonoid contents of MEGWDS were evaluated using standard techniques. In vitro antioxidant and antidiabetic potentials were determined using the DPPH radical scavenging assay and α-amylase inhibitory assay, respectively. In vivo antidiabetic activity was assessed in normoglycemic and alloxan-induced diabetic rats. Induction of diabetes in rats was done with a single dose of alloxan (150 mg/kg, Intraperitoneal, IP). MEGWDS (75, 150, and 300 mg/kg, per os, PO) and glibenclamide (5 mg/kg, PO) were administered in different experiments.</p> Results <p>Phytochemical analysis of MEGWDS showed the presence of alkaloids, flavonoids, saponins, tannins, carbohydrates and phenols. Total phenolic and flavonoid contents were 19.892 ± 2.819 mg/g GAE and 4.906 ± 1.577 mg/g QE, respectively. DPPH assay showed significant antioxidant activity (IC<sub>50</sub>= 87.94 µg/mL). The α-amylase inhibitory assay showed significant antidiabetic activity (IC<sub>50</sub>= 45.26 µg/mL). Oral glucose tolerance test (OGTT) with MEGWDS depicted 36.98% and 56.2% decrease in blood glucose levels (BGLs) in normoglycemic and diabetic rats, respectively. MEGWDS showed a significant dose-dependent antihyperglycemic effect in acute and sub-acute studies in diabetic rats. Liver function tests (LFTs) and renal function tests (RFTs) showed a significant decrease in biochemical markers in MEGWDS-treated groups. MEGWDS-treated groups exhibited a significant reduction in glycosylated hemoglobin (HbA1c) level. </p> Conclusion <p>It was concluded that MEGWDS showed a significant antidiabetic effect in diabetic rats. This study justifies the traditional claim for the use of GWDS in diabetes.</p>

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Phytochemical Analysis, Antioxidant and Pharmacological Evaluation of Antidiabetic Activity of Methanolic Extract of Geranium wallichianum D. Don ex Sweet in Alloxan-Induced Diabetic Rats

  • 期刊论文

  • 发表日期：

    2026年6月15日

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  • Fatima Afzal,
  • Abdul Aleem,
  • Javed Iqbal,
  • Fahad Asim,
  • Irfan Anjum,
  • Muhammad Naveed Mushtaq

摘要

Purpose

The current study was conducted to assess the qualitative phytochemical profile, antioxidant, and antidiabetic effect of methanolic extract of GWDS (MEGWDS).

Methods

Phytochemical constituents, total phenolic, and flavonoid contents of MEGWDS were evaluated using standard techniques. In vitro antioxidant and antidiabetic potentials were determined using the DPPH radical scavenging assay and α-amylase inhibitory assay, respectively. In vivo antidiabetic activity was assessed in normoglycemic and alloxan-induced diabetic rats. Induction of diabetes in rats was done with a single dose of alloxan (150 mg/kg, Intraperitoneal, IP). MEGWDS (75, 150, and 300 mg/kg, per os, PO) and glibenclamide (5 mg/kg, PO) were administered in different experiments.

Results

Phytochemical analysis of MEGWDS showed the presence of alkaloids, flavonoids, saponins, tannins, carbohydrates and phenols. Total phenolic and flavonoid contents were 19.892 ± 2.819 mg/g GAE and 4.906 ± 1.577 mg/g QE, respectively. DPPH assay showed significant antioxidant activity (IC50= 87.94 µg/mL). The α-amylase inhibitory assay showed significant antidiabetic activity (IC50= 45.26 µg/mL). Oral glucose tolerance test (OGTT) with MEGWDS depicted 36.98% and 56.2% decrease in blood glucose levels (BGLs) in normoglycemic and diabetic rats, respectively. MEGWDS showed a significant dose-dependent antihyperglycemic effect in acute and sub-acute studies in diabetic rats. Liver function tests (LFTs) and renal function tests (RFTs) showed a significant decrease in biochemical markers in MEGWDS-treated groups. MEGWDS-treated groups exhibited a significant reduction in glycosylated hemoglobin (HbA1c) level.

Conclusion

It was concluded that MEGWDS showed a significant antidiabetic effect in diabetic rats. This study justifies the traditional claim for the use of GWDS in diabetes.