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Purpose <p>This study investigated the <i>in vitro</i> antioxidant and anti-hemolytic activities of the crude extract (CrE) of <i>Reichardia picroides</i> L. (<i>R. picroides</i>) and its fractions, and aimed to isolate and structurally characterise a bioactive compound supported by computational analysis, including molecular docking on α-glucosidase.</p> Methods <p>This study investigated the <i>in vitro</i> antioxidant and anti-hemolytic activities of <i>R</i>. <i>picroides</i> extract and its fractions. A bioactive compound, luteolin-7-<i>O</i>-β-glucoside, was isolated and structurally characterized by NMR spectroscopy. <i>In silico</i> study included molecular docking and ADME evaluation.</p> Results <p>The ethyl acetate extract (EAE) exhibited the highest phenolic and flavonoid contents and showed pronounced antioxidant and anti-hemolytic activities. EAE significantly prolonged HT₅₀ compared with the control. Molecular docking analysis suggested favorable binding interactions between luteolin-7-<i>O</i>-β-glucoside and α-glucosidase. The DFT and ADME analyses indicated favorable pharmacokinetic properties, although experimental validation is required.</p> Conclusion <p>The findings provide preliminary evidence that <i>R. picroides</i> contains bioactive constituents with antioxidant and membrane-protective properties. The isolated luteolin-7-<i>O</i>-β-glucoside may contribute to the observed activities. However, further in vivo and mechanistic studies are required to confirm the biological relevance of these effects.</p>

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Phytochemical Insights into Reichardia picroides L.: Luteolin-7-O-β-Glucoside Isolation, In Silico Analysis, and Bioactivity of Extract and Fractions

  • 期刊论文

  • 发表日期：

    2026年7月4日

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  • Sana Aouachria,
  • Soumia Mouffok,
  • Souhila Laib,
  • Mohamed Sabri Bensaad,
  • Sabah Boumerfeg,
  • Lekhmici Arrar,
  • Abderrahmane Baghiani

摘要

Purpose

This study investigated the in vitro antioxidant and anti-hemolytic activities of the crude extract (CrE) of Reichardia picroides L. (R. picroides) and its fractions, and aimed to isolate and structurally characterise a bioactive compound supported by computational analysis, including molecular docking on α-glucosidase.

Methods

This study investigated the in vitro antioxidant and anti-hemolytic activities of R. picroides extract and its fractions. A bioactive compound, luteolin-7-O-β-glucoside, was isolated and structurally characterized by NMR spectroscopy. In silico study included molecular docking and ADME evaluation.

Results

The ethyl acetate extract (EAE) exhibited the highest phenolic and flavonoid contents and showed pronounced antioxidant and anti-hemolytic activities. EAE significantly prolonged HT₅₀ compared with the control. Molecular docking analysis suggested favorable binding interactions between luteolin-7-O-β-glucoside and α-glucosidase. The DFT and ADME analyses indicated favorable pharmacokinetic properties, although experimental validation is required.

Conclusion

The findings provide preliminary evidence that R. picroides contains bioactive constituents with antioxidant and membrane-protective properties. The isolated luteolin-7-O-β-glucoside may contribute to the observed activities. However, further in vivo and mechanistic studies are required to confirm the biological relevance of these effects.