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Purpose <p>Medicinal plants such as <i>Withania somnifera</i> (Ashwagandha), <i>Tinospora crispa</i> (Gulancha), <i>Cinnamomum zeylanicum</i> (Dalchini), and <i>Piper longum</i> (Pippali) play a vital role in traditional Indian medicine for managing oxidative stress-related conditions. This study aimed to analyze their hydroalcoholic extracts’ bioactive phytochemical profiles, quantify phenolics and flavonoids, evaluate antioxidant efficacy, and correlate these properties with therapeutic potential through spectroscopic analysis.</p> Methods <p>Dried plant parts underwent cold maceration with hydroalcoholic solvent to prepare extracts. Qualitative screening detected secondary metabolites (tannins, phenolics, alkaloids, glycosides) using standard assays. Quantitative analysis was performed to measure Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). Antioxidant activity was assessed via DPPH and H₂O₂ scavenging assays, malondialdehyde (MDA) reduction for lipid peroxidation inhibition, and Fourier Transform Infrared (FTIR) spectroscopy for functional group identification.</p> Results <p>Extracts confirmed the presence of tannins, phenolics, alkaloids, and glycosides, with high TPC and TFC indicating substantial antioxidant potential. DPPH and H₂O₂ assays showed strong free radical scavenging; MDA levels significantly reduced, demonstrating protection against oxidative damage. FTIR revealed key functional groups (e.g., O-H, C = O) linked to bioactivity, highest in Ashwagandha and Pippali extracts.</p> Conclusion <p>Hydroalcoholic extracts of these plants exhibit robust antioxidant properties, scientifically validating their traditional use in Indian ethnomedicine. Integration of phytochemical, antioxidant, and spectroscopic data underscores their promise as natural therapeutic agents for oxidative stress, particularly in resource-limited settings, paving the way for plant-based drug development.</p>

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FTIR-Guided Antioxidant Profiling of Selected Indian Botanicals for Potential Application in Oxidative Stress Management

  • 期刊论文

  • 发表日期：

    2026年5月21日

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  • Rohit Pal,
  • Bhaskar Choudhury

摘要

Purpose

Medicinal plants such as Withania somnifera (Ashwagandha), Tinospora crispa (Gulancha), Cinnamomum zeylanicum (Dalchini), and Piper longum (Pippali) play a vital role in traditional Indian medicine for managing oxidative stress-related conditions. This study aimed to analyze their hydroalcoholic extracts’ bioactive phytochemical profiles, quantify phenolics and flavonoids, evaluate antioxidant efficacy, and correlate these properties with therapeutic potential through spectroscopic analysis.

Methods

Dried plant parts underwent cold maceration with hydroalcoholic solvent to prepare extracts. Qualitative screening detected secondary metabolites (tannins, phenolics, alkaloids, glycosides) using standard assays. Quantitative analysis was performed to measure Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). Antioxidant activity was assessed via DPPH and H₂O₂ scavenging assays, malondialdehyde (MDA) reduction for lipid peroxidation inhibition, and Fourier Transform Infrared (FTIR) spectroscopy for functional group identification.

Results

Extracts confirmed the presence of tannins, phenolics, alkaloids, and glycosides, with high TPC and TFC indicating substantial antioxidant potential. DPPH and H₂O₂ assays showed strong free radical scavenging; MDA levels significantly reduced, demonstrating protection against oxidative damage. FTIR revealed key functional groups (e.g., O-H, C = O) linked to bioactivity, highest in Ashwagandha and Pippali extracts.

Conclusion

Hydroalcoholic extracts of these plants exhibit robust antioxidant properties, scientifically validating their traditional use in Indian ethnomedicine. Integration of phytochemical, antioxidant, and spectroscopic data underscores their promise as natural therapeutic agents for oxidative stress, particularly in resource-limited settings, paving the way for plant-based drug development.