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<p><i>Getonia floribunda</i> Roxb extract (GFE), a traditional medicinal plant known for its anthelminthic, antiviral, anti-inflammatory, antioxidant and wound-healing properties has been less explored. The current study, aimed to extract and evaluate the bioactive phytoconstituents such as flavonoids, phenols, diterpenes and others by using petroleum ether, chloroform, ethyl acetate and methanol. While, antioxidant assay using DPPH and FRAP methods demonstrated relatively lesser IC<sub>50</sub> value of 115&#xa0;µg/mL and 304&#xa0;µg/mL respectively, as compared to standards. Moderate antibacterial activity was observed against clinical isolates of <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, <i>Proteus mirabilis</i> and <i>Escherichia coli</i> with methanolic extract (50&#xa0;mg/mL) on agar diffusion plate. In addition, GFE extract showed differential inhibitory effect on Multidrug resistant (MDR) <i>S. aureus</i>, although exceptional inhibition against <i>Enterococcus faecalis</i> was observed with minimal of 3.75&#xa0;mg/mL. <i>P. mirabilis</i> and <i>P. aeruginosa</i> showed sensitivity to GFE at 15&#xa0;mg/mL. GC–MS profiling identified mainly 43 compounds in GFE, including n-Hexadecanoic acid, 9-Octadecenoic acid, 1,2,3-propanetriyl ester, (E, E,E), Octadecanoic acid, gamma. -Sitosterol and Z, ZZ, Z-11,13-Hexadecadien-1-ol acetate. Subsequently, FTIR confirmed the presence of polyphenols, aliphatic compounds, fatty acid esters, inorganic carbonate, oligosaccharides etc. Our present study, highlights for the first time remarkable potential of GFE for its antibacterial properties against MDR clinical isolates with colistin resistant, which signifies much better over previously reported phytoconstituents. Enhanced antibacterial potency was observed for the methanolic extract, with effective inhibition of clinical isolates at 50&#xa0;mg/mL whereas, 100&#xa0;mg/mL has been reported for non-clinical strains in various reference studies. Succeeding with significant inhibition against CRE-<i>E. coli</i> (Carbapenem resistant Enterobacteriales - <i>E. coli</i>) and <i>Acinetobacter baumannii</i> at 60&#xa0;mg/mL, GFE exhibits an effective promising antibacterial potential.</p> Graphical Abstract <p></p>

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Evaluation of Getonia floribunda Roxb Extracts (GFE) for Phytoconstituents and Bacterial Toxicity Against Multidrug Resistant (MDR) Clinical Isolates

  • 期刊论文

  • 发表日期：

    2026年4月18日

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  • Sahana M.,
  • Ganapati Bhat,
  • Manjula Nagalapur Gadilingappa

摘要

Getonia floribunda Roxb extract (GFE), a traditional medicinal plant known for its anthelminthic, antiviral, anti-inflammatory, antioxidant and wound-healing properties has been less explored. The current study, aimed to extract and evaluate the bioactive phytoconstituents such as flavonoids, phenols, diterpenes and others by using petroleum ether, chloroform, ethyl acetate and methanol. While, antioxidant assay using DPPH and FRAP methods demonstrated relatively lesser IC50 value of 115 µg/mL and 304 µg/mL respectively, as compared to standards. Moderate antibacterial activity was observed against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis and Escherichia coli with methanolic extract (50 mg/mL) on agar diffusion plate. In addition, GFE extract showed differential inhibitory effect on Multidrug resistant (MDR) S. aureus, although exceptional inhibition against Enterococcus faecalis was observed with minimal of 3.75 mg/mL. P. mirabilis and P. aeruginosa showed sensitivity to GFE at 15 mg/mL. GC–MS profiling identified mainly 43 compounds in GFE, including n-Hexadecanoic acid, 9-Octadecenoic acid, 1,2,3-propanetriyl ester, (E, E,E), Octadecanoic acid, gamma. -Sitosterol and Z, ZZ, Z-11,13-Hexadecadien-1-ol acetate. Subsequently, FTIR confirmed the presence of polyphenols, aliphatic compounds, fatty acid esters, inorganic carbonate, oligosaccharides etc. Our present study, highlights for the first time remarkable potential of GFE for its antibacterial properties against MDR clinical isolates with colistin resistant, which signifies much better over previously reported phytoconstituents. Enhanced antibacterial potency was observed for the methanolic extract, with effective inhibition of clinical isolates at 50 mg/mL whereas, 100 mg/mL has been reported for non-clinical strains in various reference studies. Succeeding with significant inhibition against CRE-E. coli (Carbapenem resistant Enterobacteriales - E. coli) and Acinetobacter baumannii at 60 mg/mL, GFE exhibits an effective promising antibacterial potential.

Graphical Abstract