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<p>Triple negative breast cancer (TNBC) is aggressive subtype of breast cancer and has minimal treatment therapy. In the present work, we have developed a green and environmentally friendly synthesis of, Tranilast loaded silver nanoparticle (TRL-AgNPs), using an aqueous leaf extract of <i>Ocimum sanctum</i> (Holy Basil) as the capping, stabilizing and reducing agent. The prepared formulations were optimized by employing Box–Behnken design (BBD) via Design-Expert® software to obtain an ideal formulation (Run 14) with particle size 100.56 ± 4.12&#xa0;nm, zeta potential − 28.21 ± 1.13 mV and entrapment efficiency 87.6 ± 3.5%. Additionally, these TRL-AgNPs were integrated into a triblock thermosensitive polymeric PLEL hydrogel which shows sol-gel transitional behaviour. with a sustained release of drugs of approximately 90% during 14 days. For characterization UV-Vis, DLS, XRD, TEM, SEM and zeta potential were used to ascertain the stability and structural integrity. In vitro cytotoxicity studies on on MDA-MB-231 cells demonstrated dose-dependent response with the IC<sub>50</sub> of 54.09&#xa0;µg/mL of TRL-AgNPs-HG mediated through the apoptosis, and G1/S phase cell cycle arrest. The hydrogel based delivery system facilitated local delivery while reducing acute cytotoxicity. These results indicate that there is a potential of a promising biocompatible platform for targeted TNBC therapy offering increased efficacy and reduced systemic toxicity.</p>

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Optimization of Ocimum sanctum-Mediated Tranilast-Loaded Silver Nanoparticles in a Thermosensitive PDLLA-PEG-PDLLA Hydrogel: Green Synthesis, Characterization, and Cytotoxic Efficacy Against Triple-Negative Breast Cancer Cells

  • 期刊论文

  • 发表日期：

    2026年4月14日

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  • Shraddha Ram,
  • Anil T. Pawar,
  • Amol A. Tagalpallewar,
  • Akshay M. Baheti

摘要

Triple negative breast cancer (TNBC) is aggressive subtype of breast cancer and has minimal treatment therapy. In the present work, we have developed a green and environmentally friendly synthesis of, Tranilast loaded silver nanoparticle (TRL-AgNPs), using an aqueous leaf extract of Ocimum sanctum (Holy Basil) as the capping, stabilizing and reducing agent. The prepared formulations were optimized by employing Box–Behnken design (BBD) via Design-Expert® software to obtain an ideal formulation (Run 14) with particle size 100.56 ± 4.12 nm, zeta potential − 28.21 ± 1.13 mV and entrapment efficiency 87.6 ± 3.5%. Additionally, these TRL-AgNPs were integrated into a triblock thermosensitive polymeric PLEL hydrogel which shows sol-gel transitional behaviour. with a sustained release of drugs of approximately 90% during 14 days. For characterization UV-Vis, DLS, XRD, TEM, SEM and zeta potential were used to ascertain the stability and structural integrity. In vitro cytotoxicity studies on on MDA-MB-231 cells demonstrated dose-dependent response with the IC50 of 54.09 µg/mL of TRL-AgNPs-HG mediated through the apoptosis, and G1/S phase cell cycle arrest. The hydrogel based delivery system facilitated local delivery while reducing acute cytotoxicity. These results indicate that there is a potential of a promising biocompatible platform for targeted TNBC therapy offering increased efficacy and reduced systemic toxicity.