Scholarlink

Background <p>Carvedilol (CAR) is a non-selective beta-blocker used in treating hypertension and heart failure. Existing analytical methods for CAR quantification face challenges in sensitivity, environmental sustainability, and efficiency.</p> Objectives <p>This study aimed to develop an innovative, highly sensitive, and environmentally friendly analytical method for picoscale determination of CAR in pharmaceutical formulations and biological samples.</p> Method <p>The research utilized a microwave-assisted micro-level Mannich-type derivatization to generate a CAR-linked fluorescent biosensing probe on TLC plates. The method development integrated principles of white analytical chemistry and Quality by Design (QbD) to optimize performance and minimize environmental impact.</p> Results <p>The developed method demonstrated exceptional sensitivity with detection and quantification limits in the picogram range (LOD: 3.0 pg/band, LOQ: 10 pg/band), surpassing conventional techniques. It showed high accuracy and precision in both pharmaceutical formulations (99.69-100.55% recovery) and biological samples (91.42–96.50% recovery in human plasma). The method generated zero organic waste and consumed minimal resources, achieving higher scores on analytical profile tools compared to conventional methods.</p> Conclusion <p>This research presents a significant advancement in CAR analysis, offering a highly sensitive, eco-friendly, and efficient analytical tool that balances analytical performance with environmental responsibility and user-friendliness, addressing key limitations of traditional analytical approaches.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Sensitive and Selective Fluorescent Mannich Adduct Biosensing Probe for Estimation of Carvedilol Using Integrated Approach of White Analytical Chemistry and Quality by Design

  • 期刊论文

  • 发表日期：

    2026年1月23日

查看全文
  • Pintu Prajapati,
  • Neha Nhavi,
  • Anzarul Haque,
  • Sarfaraz Ahmad,
  • Shailesh Shah

摘要

Background

Carvedilol (CAR) is a non-selective beta-blocker used in treating hypertension and heart failure. Existing analytical methods for CAR quantification face challenges in sensitivity, environmental sustainability, and efficiency.

Objectives

This study aimed to develop an innovative, highly sensitive, and environmentally friendly analytical method for picoscale determination of CAR in pharmaceutical formulations and biological samples.

Method

The research utilized a microwave-assisted micro-level Mannich-type derivatization to generate a CAR-linked fluorescent biosensing probe on TLC plates. The method development integrated principles of white analytical chemistry and Quality by Design (QbD) to optimize performance and minimize environmental impact.

Results

The developed method demonstrated exceptional sensitivity with detection and quantification limits in the picogram range (LOD: 3.0 pg/band, LOQ: 10 pg/band), surpassing conventional techniques. It showed high accuracy and precision in both pharmaceutical formulations (99.69-100.55% recovery) and biological samples (91.42–96.50% recovery in human plasma). The method generated zero organic waste and consumed minimal resources, achieving higher scores on analytical profile tools compared to conventional methods.

Conclusion

This research presents a significant advancement in CAR analysis, offering a highly sensitive, eco-friendly, and efficient analytical tool that balances analytical performance with environmental responsibility and user-friendliness, addressing key limitations of traditional analytical approaches.