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Background <p>Rheumatoid arthritis (RA) is an autoimmune chronic illness affecting 0.5% to 1% of the population. Traditional medicinal plants, which are pivotal in drug discovery, prompted the investigation of <i>Pueraria tuberosa</i> (PT) tuber for its anti-rheumatic properties.</p> Purpose <p>Aqueous extract of PT and <i>Pueraria tuberosa</i> loaded silver nanoparticle (PTAgNP) were investigated for their effects in Freund’s Complete Adjuvant induced RA in rats.</p> Methods <p>In vivo evaluation using the Freund’s complete adjuvant (FCA) induced arthritis model and <i>Pueraria tuberosa</i> tuber extract at a dose (200, 400&#xa0;mg/kg) and PTAgNP 100&#xa0;mg/kg was used as a treatment for about 21 days. NPs were characterized using UV, XRD, SEM, and zetasizer. After 21 days of treatment, oxidative stress in paw tissue, biochemical parameters, inflammatory cytokines, x-ray, and histopathological analysis of the ankle joint were evaluated.</p> Results <p>PT and PTAgNP showed treatment significantly ameliorates the adjuvant induced arthritic scoring, histological alterations, paw volume, elevation of biochemical (AST, ALT, ALP, CRP) and restored the endogenous anti-oxidant (SOD, GSH, MDA) activities. Significant reduction in paw swelling, arthritis score and weight of spleen in treatment groups. An Increased in body weight, indicating improvement of the disease condition. Biochemical analyses indicated reduced ESR, WBC and increased levels of RBC and Hb, suggesting decreased inflammation. There was also a significant decrease in levels of TNF-alpha and IL-6 pro-inflammatory cytokines in treatment groups. Radiological examination showed reduced soft tissue swelling and joint changes in the treated groups.</p> Conclusion <p>PT and PTAgNPs showed anti-arthritic effects through anti-oxidant activity, reduction of inflammatory markers and improvement in joint parameters. These findings support further exploration of PT and the nanoparticles demonstrated significant antiarthritic activity against rheumatoid arthritis induced by complete Freund’s adjuvant in experimental rats, surpassing the effects of the extract and standard indomethacin. <i>Pueraria tuberosa</i> loaded silver nanoparticles showed as potential source as a novel anti-arthritic drug.</p>

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Green-Synthesis and Characterization of Pueraria Tuberosa Aqueous Extract Loaded Silver Nanoparticles for Antiarthritic Effect Against Freund’s Complete Adjuvant Induced in Male Wistar Rats

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • Tejas Nirwane,
  • Namit Kudatarkar

摘要

Background

Rheumatoid arthritis (RA) is an autoimmune chronic illness affecting 0.5% to 1% of the population. Traditional medicinal plants, which are pivotal in drug discovery, prompted the investigation of Pueraria tuberosa (PT) tuber for its anti-rheumatic properties.

Purpose

Aqueous extract of PT and Pueraria tuberosa loaded silver nanoparticle (PTAgNP) were investigated for their effects in Freund’s Complete Adjuvant induced RA in rats.

Methods

In vivo evaluation using the Freund’s complete adjuvant (FCA) induced arthritis model and Pueraria tuberosa tuber extract at a dose (200, 400 mg/kg) and PTAgNP 100 mg/kg was used as a treatment for about 21 days. NPs were characterized using UV, XRD, SEM, and zetasizer. After 21 days of treatment, oxidative stress in paw tissue, biochemical parameters, inflammatory cytokines, x-ray, and histopathological analysis of the ankle joint were evaluated.

Results

PT and PTAgNP showed treatment significantly ameliorates the adjuvant induced arthritic scoring, histological alterations, paw volume, elevation of biochemical (AST, ALT, ALP, CRP) and restored the endogenous anti-oxidant (SOD, GSH, MDA) activities. Significant reduction in paw swelling, arthritis score and weight of spleen in treatment groups. An Increased in body weight, indicating improvement of the disease condition. Biochemical analyses indicated reduced ESR, WBC and increased levels of RBC and Hb, suggesting decreased inflammation. There was also a significant decrease in levels of TNF-alpha and IL-6 pro-inflammatory cytokines in treatment groups. Radiological examination showed reduced soft tissue swelling and joint changes in the treated groups.

Conclusion

PT and PTAgNPs showed anti-arthritic effects through anti-oxidant activity, reduction of inflammatory markers and improvement in joint parameters. These findings support further exploration of PT and the nanoparticles demonstrated significant antiarthritic activity against rheumatoid arthritis induced by complete Freund’s adjuvant in experimental rats, surpassing the effects of the extract and standard indomethacin. Pueraria tuberosa loaded silver nanoparticles showed as potential source as a novel anti-arthritic drug.