Optimization of Tissue Culture Media for Microtuber Development Incorporating Sugars and Zinc Nanoparticles
摘要
This study deals with the long term problem of seed tuber scarcity to plant potato. The given work puts an emphasis on the effects of sucrose, cytokinins and zinc nanoparticles (ZnNPs) on potato microtuberization. The optimum level was screened by using different levels (3%, 5%, 6%, 7%, and 8%) of sucrose in Murashige and Skoog media. 6% sucrose emerged as the most effective concentration and was used in combination with varying concentrations of cytokinins (6-Benzylaminopurine and Kinetin). Cytokinins did not exhibit any significant improvement in microtuberization. The study focused on investigating the effect of ZnNPs in combination with different concentrations of sucrose on the microtuberization. It was found out that 40 mg kg− 1 ZnNPs and 6% sucrose resulted in the maximum number of microtubers, that is 5.33 ± 0.67 microtubers within 14 days having average tuber weight of 0.70 g. Biochemical analysis was carried out to check the influence of ZnNPs on various parameters. The recorded increment in total soluble protein contents is directly correlated with microtuber formation and growth. Similarly, at the time of microtuber induction total soluble sugar contents, particularly non-reducing sugars were higher than reducing sugar. Specific activity of nitrate reductase and enzymes such as phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase were also enhanced in response to microtuberization. This analysis provided insights into the biochemical changes occurring after application of ZnNPs.