<p>The current investigation aimed to highlight the potential of probiotics isolated from lactic acid bacteria of different Algerian ecosystems as antioxidant and antidiabetic agents. Fifty-four strains from different ecosystems were isolated anaerobically. The subsequent steps involved comprehensive characterization and in vitro assessments, including assays for scavenging DPPH and ABTS•+ radicals, measuring ferric ion reducing power (FRAP), and assessing the impact of the strains on the α-amylase enzyme. Additionally, the hydrophobicity potential, aggregation and coaggregation capacities, antibacterial activity, and absence of hemolytic activity were investigated. Ten (10) out of the fifty-four (54) strains exhibited characteristics of probiotics like resistance at a low gastric pH (2.0–3.0) (&gt; 55% survival rate) and survival under different concentrations of NaCl and bile salts 0.3%-1% (&gt; 80% survival rate). All the isolated strains exhibited high hydrophobicity potential, remarkable aggregation and coaggregation capacities, and antibacterial activity but no hemolytic activity. Because of the antioxidant capacity of the selected strains, every strain seems to be capable of efficiently scavenging DPPH and ABTS•+ radicals, superoxide dismutase and hydroxyl radical scavenging activity, and high resistance to H₂O₂. The findings of this study strongly suggest that the identified strains hold significant promise as potential probiotic candidates. Their observed capabilities make them particularly valuable in addressing oxidative stress and associated conditions, such as diabetes.</p> Graphical Abstract <p></p>