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<p>A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820<sup>T</sup>, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0–9.0 (optimum pH 7.0), at 24–39&#xa0;°C (optimum 37&#xa0;°C) and with 0.5–2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to <i>Acinetobacter kanungonis</i> JCM 34131<sup>T</sup>. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (<i>Acinetobacter calcoaceticus</i> DSM 30006<sup>T</sup>), 37.0% (<i>Fluviicoccus keumensis</i> JCM 19370<sup>T</sup>), and 64.0% (<i>Acinetobacter kanungonis</i> JCM 34131<sup>T</sup>), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C<sub>12:0</sub>, C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub>ω7c and/ or C<sub>16:1</sub>ω6c), C<sub>17:0</sub>, and C<sub>15:1</sub>ω6c as the major fatty acid (&gt; 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820<sup>T</sup> represents a novel species of a new genus in the family Moraxellaceae, and the name <i>Nasibacterium caprae</i> gen. nov., sp. nov. is proposed. The type strain is ZY210820<sup>T</sup> (= CCTCC AB 2021475<sup>T</sup> = NBRC 115474<sup>T</sup>).</p>

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Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease

  • 期刊论文

  • 发表日期：

    2025年6月6日

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  • Fuxiang Li,
  • Huafeng Gao,
  • Wenhua Zhao,
  • Jianling Song

摘要

A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820T, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0–9.0 (optimum pH 7.0), at 24–39 °C (optimum 37 °C) and with 0.5–2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131T. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006T), 37.0% (Fluviicoccus keumensis JCM 19370T), and 64.0% (Acinetobacter kanungonis JCM 34131T), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C12:0, C16:0, summed feature 3 (C16:1ω7c and/ or C16:1ω6c), C17:0, and C15:1ω6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820T represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820T (= CCTCC AB 2021475T = NBRC 115474T).