<p>Cut-resistant textiles are essential for ensuring personal safety in industries where exposure to sharp tools and mechanical hazards is common. Despite advancements, achieving an optimal balance between protection and wearer comfort remains a significant challenge. In this study, six different single jersey and double jersey knitted fabric structures were developed using 100% ultra-high molecular weight polyethylene (UHMWPE) yarn to address the need for flexible and effective cut-resistant textiles. The fabrics were produced using a 14-gauge V-bed flat knitting machine with variations in loop configuration, including knit, tuck, and float stitches. The physical, mechanical, and comfort-related properties of the samples were systematically evaluated. Structural parameters such as thickness, areal density, and stitch density showed significant influence on cut, puncture, tear, and abrasion resistance properties. Rib-based fabrics provided the highest level of mechanical protection due to their compact and interlocked loop arrangement, while plain-knitted fabrics showed moderate mechanical protection with better air and moisture permeability, offering higher comfort and suitability for long-term wear. Fabrics with float stitches improved thermal insulation by trapping air within the structure, whereas tuck stitches increased breathability but slightly reduced mechanical performance. These findings highlight a structure-based design approach using 100% UHMWPE-knitted fabrics, where variations in stitch type and fabric topology are strategically applied to achieve both high protection and comfort without relying on hybrid reinforcement or surface coatings, providing guidance for the design of safer and more wearable protective clothing.</p>