<p>Bacterial cellulose (BC) is well-known as a new alternative material in the textile and leather industry; however, its limitations in comfort performance in the dehydrated state hinder its attraction in this field. This study presents a modification approach to enhance the comfort performance of dehydrated BC through the incorporation of recycled silk fibroin and electron beam irradiation (EBI). In terms of sensorial comfort, the integration of fibroin gel effectively mitigates the inherent rigidity and brittleness of BC by forming a stable interpenetrating polymer network. This structural modification results in a substantial reduction in bending modulus, from 3478.2 ± 360.2&#xa0;MPa of pristine BC to 652.7 ± 194.0&#xa0;MPa of the BC/Fibroin composite, accompanied by an increase in tensile strain from 2.3 ± 0.9 to 5.8 ± 1.6%. Subsequent EBI treatment further optimizes these properties, yielding an EBI-BC/Fibroin composite with a markedly lower bending modulus of 43.6 ± 14.7&#xa0;MPa and an enhanced tensile strain of 8.8 ± 2.9%. The EBI-induced cross-linking also improves structural integrity, ensuring the stable retention of fibroin gel after rinsing by acetone and resulting in a higher areal density (190.6 ± 33.5&#xa0;g/m²) compared to BC (49.1 ± 0.9&#xa0;g/m²). Furthermore, the synergistic modification (EBI-BC/Fibroin) enhances thermal comfort, as reflected by an elevated <i>Q</i>_max value of 0.237 ± 0.010&#xa0;W/cm², compared to 0.081 ± 0.005&#xa0;W/cm² of BC. These findings demonstrate the potential of combining recycled protein resources and irradiation processing to tailor comfort properties of BC-based materials for textile and leather applications. </p> Graphical Abstract <p></p>