<p>Sustainability has gained attention due to its role in addressing environmental issues in recent years. Natural fibers, especially jute, have enough potential to become an essential reinforcing element in fiber-reinforced latex composites. To achieve a high puncture resistance in jute composites, woven structures are considered as load-bearing preforms with varying dimensions. Various woven structures, including two-dimensional (2D), 2.5-dimensional (2.5D), and three-dimensional (3D), are considered to study the effect of dimension and to optimize other structural parameters of woven-jute/latex (WJL) composites. Compression molding was performed after a hand layup procedure to prepare WJL composites. Scanning electron microscopy (SEM) analysis was employed to validate the penetration of latex inside the 2D, 2.5D, and 3D woven structures. The reinforcing efficiencies of the 2D, 2.5D, and 3D woven fabrics were compared, and it was found that the fiber arrangement inside the woven structure largely influenced shore hardness and puncture resistance. The puncture resistance of jute-latex composites was 300.2 N with 2D jute-based preforms and enhanced to 839N with 3D orthogonal jute-based preforms, and the shore hardness value was improved from 65 in 2D to 84.5 with 3D orthogonal preforms. The performance of the 2.5D jute-based preform fell between that of 2D and 3D jute-based preforms. This research will provide a solid foundation for introducing different woven structures in WJL composites for various composite products. With a higher correlation coefficient, the statistical model's suitability and correctness were validated. Indicating that the experimental results align with the statistical model, stronger correlation coefficients (R²) were found for both puncture (0.977) and hardness (0.877).</p>