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<p>Exposure to acute and prolonged microgravity triggers numerous physiological adaptations. To date, the underlying molecular mechanisms are not well understood, and several pathways have been proposed. Among other candidates, specific ion channels are hypothesized to be gravity dependent, but it has not been possible to conclusively demonstrate gravity dependency of specific protein entities. Therefore, we developed a miniaturized two-electrode voltage clamp (TEVC) that allowed electrophysiological experiments on <i>Xenopus laevis</i> oocytes using the GraviTower Bremen Prototype (GTB-Pro). The GTB-Pro is capable of flying experiments on a vertical parabolic trajectory, providing microgravity for a few seconds. As an interesting first candidate, we examined whether the nonselective mechanosensitive ion channel PIEZO1 is gravity dependent. The results showed no difference between PIEZO1-overexpressing and control oocytes under acute microgravity conditions.</p>

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Recordings on PIEZO1-Overexpressing Oocytes in Microgravity

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月6日

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  • Simon L. Wuest,
  • Geraldine Cerretti,
  • Jennifer Polzer,
  • Simon Gerig,
  • Christoph Zumbühl,
  • Christian Jost,
  • Lukas Rüfenacht,
  • Robert Eberli,
  • Barbara Krucker-Bösch,
  • Julia Traversari,
  • Melanie Horn,
  • Daniel Invernot Pérez,
  • Christina Giger-Lange,
  • Karin F. Rattenbacher-Kiser,
  • Fabian Ille,
  • Gerhard Székely,
  • Soeren S. Lienkamp,
  • Marcel Egli

摘要

Exposure to acute and prolonged microgravity triggers numerous physiological adaptations. To date, the underlying molecular mechanisms are not well understood, and several pathways have been proposed. Among other candidates, specific ion channels are hypothesized to be gravity dependent, but it has not been possible to conclusively demonstrate gravity dependency of specific protein entities. Therefore, we developed a miniaturized two-electrode voltage clamp (TEVC) that allowed electrophysiological experiments on Xenopus laevis oocytes using the GraviTower Bremen Prototype (GTB-Pro). The GTB-Pro is capable of flying experiments on a vertical parabolic trajectory, providing microgravity for a few seconds. As an interesting first candidate, we examined whether the nonselective mechanosensitive ion channel PIEZO1 is gravity dependent. The results showed no difference between PIEZO1-overexpressing and control oocytes under acute microgravity conditions.