<p>This study aimed to clarify the influence of different breath-holding techniques on craniocaudal misregistration between the arterial and venous phases in preoperative abdominal contrast-enhanced computed tomography. A retrospective analysis was conducted on 60 patients who underwent contrast-enhanced CT for preoperative pancreatic evaluation, with 30 using inspiration breath-holding and 30 using expiration breath-holding. Craniocaudal differences in the start and end slice positions of the liver, pancreas, spleen, and kidneys were measured between the arterial and venous phases. Two observers performed a five-point visual evaluation of misregistration in the liver, pancreas, spleen, kidneys, celiac artery, splenic artery, and common hepatic artery. Quantitatively, only the starting slice position of the liver showed a significantly smaller difference in the inspiration group (3.3 mm vs. 2.3 mm; p &lt; 0.05). No significant differences were observed in other organs. These findings suggest that breath-holding technique does not significantly affect craniocaudal misregistration between the arterial and venous phases in preoperative abdominal CT.</p>