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<p>Food adulteration involving peach components on the market poses a significant allergic risk, necessitating the development of reliable detection strategies. In this study, a rapid detection method of peach (<i>Prunus persica</i>) components was established by combining loop-mediated isothermal amplification (LAMP) with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12b system, targeting the specific gene sequence of peach allergen coding gene <i>Pru p</i> 2.01B. This integration leverages CRISPR for both specific target recognition and auxiliary signal amplification, resulting in superior specificity and sensitivity over LAMP alone. After optimization, the qualitative detection of peach adulteration can be finished in just 50&#xa0;min at a steady 65&#xa0;°C. The sensitivity of the assay can reach 10<sup>–1</sup>&#xa0;ng/µL and is able to spot 1% (w/w) contamination, which can satisfy the detection requirements of on-site detection. When testing 17 commercially available samples, the results from our method were 100% consistent with both real-time PCR and the information declared on the product labels. This method does not rely on large-scale instrumentation, and the results can be read visually. It offers the advantages of high specificity, high sensitivity, and easy operation, providing a new technique for the rapid detection of allergenic peach components.</p>

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Rapid Detection of Peach (Prunus persica) Components in Highly Processed Foods Based on LAMP-CRISPR/Cas12b System

  • 期刊论文

  • 发表日期：

    2026年4月1日

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  • Shuangshuang Song,
  • Xin Yuan,
  • Yaru Zhu,
  • Ranran Xing,
  • Jiukai Zhang,
  • Ying Chen,
  • Tingting Deng,
  • Qian Li

摘要

Food adulteration involving peach components on the market poses a significant allergic risk, necessitating the development of reliable detection strategies. In this study, a rapid detection method of peach (Prunus persica) components was established by combining loop-mediated isothermal amplification (LAMP) with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12b system, targeting the specific gene sequence of peach allergen coding gene Pru p 2.01B. This integration leverages CRISPR for both specific target recognition and auxiliary signal amplification, resulting in superior specificity and sensitivity over LAMP alone. After optimization, the qualitative detection of peach adulteration can be finished in just 50 min at a steady 65 °C. The sensitivity of the assay can reach 10–1 ng/µL and is able to spot 1% (w/w) contamination, which can satisfy the detection requirements of on-site detection. When testing 17 commercially available samples, the results from our method were 100% consistent with both real-time PCR and the information declared on the product labels. This method does not rely on large-scale instrumentation, and the results can be read visually. It offers the advantages of high specificity, high sensitivity, and easy operation, providing a new technique for the rapid detection of allergenic peach components.