Scholarlink

<p>A three-phase liquid extraction (TLPE) approach was developed and validated for the simultaneous determination of phthalates and bisphenols in seafood using gas chromatography–mass spectrometry (GC–MS). The most optimized TLPE system consisted of a mixture of hexane, dimethylformamide, and water added with Na<sub>2</sub>SO<sub>4</sub> salt in a volume ratio of 2:4:4 (v/v/v), under neutral pH conditions at room temperature. The method achieved limits of detection ranging from 0.0071 to 0.0330&#xa0;μg&#xa0;g⁻<sup>1</sup> for six phthalates and from 0.0400 to 0.0513&#xa0;μg&#xa0;g⁻<sup>1</sup> for two bisphenols in mussel matrices. Recoveries in spiked mussel, shrimp, fish, and squid samples were found to be within the ranges of 68.1–73.1%, 63.4–69.0%, 62.1–69.5%, and 62.5–68.4%, respectively, with relative standard deviations below 3%. Inter-day precision values were 1.03–2.62%. Analysis of real samples demonstrated the presence of di(2-ethylhexyl) phthalate (DEHP) as a matrix contaminant in most samples, ranging from 0.0575–0.1125&#xa0;μg&#xa0;g<sup>−1</sup>. Dibutyl phthalate (DBP), benzyl butyl phthalate (BBP) and di-n-octyl phthalate (DnOP) were found in some samples. In comparison to other extraction methods, the proposed TLPE showed superiority in terms of simultaneous extraction in a single step, low solvent consumption, small sample amount, ease of extraction, utilization of cost-effective and widely available equipment, and reduced overall extraction time.&#xa0;&#xa0;</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and Validation of a Three Liquid Phase Extraction Method for Simultaneous Determination of Phthalates and Bisphenols in Seafood by GC–MS

  • 期刊论文

  • 发表日期：

    2026年2月27日

查看全文
  • Supatra Hasanam,
  • Wimonrut Insuan,
  • Orapin Chienthavorn

摘要

A three-phase liquid extraction (TLPE) approach was developed and validated for the simultaneous determination of phthalates and bisphenols in seafood using gas chromatography–mass spectrometry (GC–MS). The most optimized TLPE system consisted of a mixture of hexane, dimethylformamide, and water added with Na2SO4 salt in a volume ratio of 2:4:4 (v/v/v), under neutral pH conditions at room temperature. The method achieved limits of detection ranging from 0.0071 to 0.0330 μg g⁻1 for six phthalates and from 0.0400 to 0.0513 μg g⁻1 for two bisphenols in mussel matrices. Recoveries in spiked mussel, shrimp, fish, and squid samples were found to be within the ranges of 68.1–73.1%, 63.4–69.0%, 62.1–69.5%, and 62.5–68.4%, respectively, with relative standard deviations below 3%. Inter-day precision values were 1.03–2.62%. Analysis of real samples demonstrated the presence of di(2-ethylhexyl) phthalate (DEHP) as a matrix contaminant in most samples, ranging from 0.0575–0.1125 μg g−1. Dibutyl phthalate (DBP), benzyl butyl phthalate (BBP) and di-n-octyl phthalate (DnOP) were found in some samples. In comparison to other extraction methods, the proposed TLPE showed superiority in terms of simultaneous extraction in a single step, low solvent consumption, small sample amount, ease of extraction, utilization of cost-effective and widely available equipment, and reduced overall extraction time.